Mouse Selp ELISA Kit

Alternative name
Mouse CD62 antigen-like family member P ELISA Kit;Mouse granule membrane protein 140 ELISA Kit;Mouse GMP-140 ELISA Kit;Mouse leukocyte-endothelial cell adhesion molecule 3 ELISA Kit;Mouse LECAM3 ELISA Kit;Mouse platelet activation dependent granule-external membrane protein ELISA Kit;Mouse PADGEM ELISA Kit;Mouse Grmp ELISA Kit;Mouse CD62P ELISA Kit;Mouse selectin, platelet ELISA Kit;Mouse P-selectin ELISA Kit;
Catalog
E062813
species
Mouse
GeneSelp
Standard Curve
Other Species Human SELP ELISA Kit Bovine SELP ELISA Kit Rat Selp ELISA Kit Sheep SELP ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 5pg/ml
Intended UseMouse Selp ELISA Kit allows for the in vitro quantitative determination of Selp , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- P-Selectin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- P-Selectin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the P-Selectin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of P-Selectin can be calculated. Background: P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule that in humans is encoded by the SELP gene. It is located on chromosome 1q21-q24, spans>50 kb and contains 17 exons in human. It is constitutively expressed on megakaryocytes (the precursor of platelets) and endothelial cells. P-selectin plays an essential role in the initial recruitment of leukocytes (white blood cells) to the site of injury during inflammation. Ligands for P-selectin on eosinophils and neutrophils are similar sialylated, protease-sensitive, endo-beta-galactosidase-resistant structures, clearly different than those reported for E-selectin, and suggest disparate roles for P-selectin and E-selectin during recruitment during inflammatory responses.
Mouse P-selectin Protein information
Uniprot ID LYAM3_MOUSE
Uniprot AC Q01102; Q32MF1;
UniGene Mm.3337;
GeneID 20344
KEGG mmu:20344;
Mouse P-selectin Protein SEQUENCE
SEQUENCE 768 AA; 83099 MW; E5173074D2F66E68 CRC64;
MAGCPKGSWT PRLRSVILGG AQLIWFSALI SELVNQKEVA AWTYNYSTKA
YSWNNSRVFC RRHFTDLVAI QNKNEIAHLN DVIPFFNSYY WIGIRKINNK
WTWVGTNKTL TEEAENWADN EPNNKKNNQD CVEIYIKSNS APGKWNDEPC
FKRKRALCYT ASCQDMSCSN QGECIETIGS YTCSCYPGFY GPECEYVKEC
GKVNIPQHVL MNCSHPLGEF SFNSQCTFSC AEGYELDGPG ELQCLASGIW
TNNPPKCDAV QCQSLEAPPH GTMACMHPIA AFAYDSSCKF ECQPGYRARG
SNTLHCTGSG QWSEPLPTCE AIACEPPEIP IHGSMDCVPS TGTFGYNSSC
TFLCAEGFVL KGNDAIQCAD SGQWTAPAPF CEALQCPEFP VPSKAQVNCS
DPFGTLTYQS VCSFSCDEGS LLVGASVIRC LATGHWNGAP PECQAVSCAP
MLSPENGSMT CVQPLGNSTY KSTCQFMCDE GFYLSGPERL DCSPSGHWTG
TPPTCEAIKC PGIFAPEQGN LDCSHVHGEF GVGSICHFSC NEDFELLGSE
NVECTVSGRW SAPPPTCKGI TSLPAPAVRC PALTTPGQGT MSCQHHLGSF
GPNTTCYFGC KTGFTLRGAN SLRCRASGQW TAVTPMCRAV KCSELHMDTA
VAMNCSNPWG NFSYGSTCTF QCPEGQSLNG SVRATCREDG HWSDAMPTCQ
AGTLTIQEAL TYLGGAVAST TGLAVGGTLL ALLRKRLRKK DDGKCPLNPH
SHLGTYGVFT NAAYDPTP
UCSC uc007dhz.2; mouse.;

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Mouse Selp ELISA Kit