SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse oxyntomodulin. No significant cross-reactivity or interference between mouse oxyntomodulin and analogues was observed.
SensitivityThe minimum detectable dose of mouse oxyntomodulinis typically less than 0.078 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determine
Intended UseMouse OGG1 ELISA Kit allows for the in vitro quantitative determination of , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPriciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for oxyntomodulin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any oxyntomodulin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for oxyntomodulin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of oxyntomodulin bound in the initial step. The color development is stopped and the intensity of the color is measured.