SPARC ELISA KIT; Basement-membrane protein 40 ELISA KIT; BM-40 ELISA KIT; Osteonectin ELISA KIT; ON ELISA KIT; Secreted protein acidic and rich in cysteineSPARC ELISA KIT; ON ELISA KIT; BM-40 ELISA KIT; ON ELISA KIT
Intended UseMouse ON ELISA Kit allows for the in vitro quantitative determination of ON , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse ON monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.