Mouse Hcrt ELISA Kit

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  • Alternative name

    Mouse hypocretin ELISA Kit;Mouse Hcrt ELISA Kit;Mouse Hypocretin-1 ELISA Kit;Mouse Hcrt1 ELISA Kit;Mouse Hypocretin-2 ELISA Kit;Mouse Hcrt2 ELISA Kit;Mouse Ox ELISA Kit;Mouse PPOX ELISA Kit;Mouse orexin ELISA Kit;Mouse orexin A ELISA Kit;Mouse orexin B ELISA Kit;Mouse prepro-orexin ELISA Kit;

  • Catalog
    E062589
  • species
    Mouse
  • GeneHcrt
  • Other Species Human HCRT ELISA KitBovine HCRT ELISA KitCanine HCRT ELISA KitPorcine HCRT ELISA KitRat Hcrt ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 4.69pg/ml
  • Intended UseMouse Hcrt ELISA Kit allows for the in vitro quantitative determination of Hcrt , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Hcrt antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Hcrt antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Hcrt amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Hcrt can be calculated.
  • Mouse Orexin-B Protein information
  • Uniprot ID OREX_MOUSE
  • Uniprot AC O55241;
  • UniGene Mm.10096;
  • GeneID 15171
  • KEGG mmu:15171;
  • Mouse Orexin-B Protein SEQUENCE
  • SEQUENCE 130 AA; 13503 MW; D3C223F9EE835F1C CRC64;

    MNFPSTKVPW AAVTLLLLLL LPPALLSLGV DAQPLPDCCR QKTCSCRLYE

    LLHGAGNHAA GILTLGKRRP GPPGLQGRLQ RLLQANGNHA AGILTMGRRA

    GAELEPHPCS GRGCPTVTTT ALAPRGGSGV

  • UCSC uc007lmg.1; mouse.;




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