Mouse Tnfrsf11b ELISA Kit

Alternative name
Mouse osteoclastogenesis inhibitory factor ELISA Kit;Mouse osteoprotegerin ELISA Kit;Mouse OCIF ELISA Kit;Mouse Opg ELISA Kit;Mouse TR1 ELISA Kit;Mouse tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) ELISA Kit;Mouse tumor necrosis factor receptor superfamily member 11B ELISA Kit;
Catalog
E062563
species
Mouse
GeneTnfrsf11b
SpecificityNatural and recombinant mouse OPG
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 5pg/ml
Intended UseMouse Tnfrsf11b ELISA Kit allows for the in vitro quantitative determination of Tnfrsf11b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse OPG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for OPG has been precoated onto 96-well plates. Standards(NSO,E22-L401) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for OPG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse OPG amount of sample captured in plate. Background: Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis.1 OPG is considered to play an important rolein the regulation of bone resorption by modifying osteoclast differentiation.2 Osteoprotegerin is an independent riskfactor for the progression of atherosclerosis and onset of cardiovascular disease.3 It can act as a soluble factor inthe regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity.4 OPG system may play a critical role in the development of osteolytic bone disease in multiplemyeloma and that targeting this system may have therapeutic potential.5 OPG also plays a role in the vascularsystem.6
Mouse Tumor necrosis factor receptor superfamily member 11B Protein information
Uniprot ID TR11B_MOUSE
Uniprot AC O08712; O70202;
UniGene Mm.15383;
GeneID 18383
KEGG mmu:18383;
Mouse Tumor necrosis factor receptor superfamily member 11B Protein SEQUENCE
SEQUENCE 401 AA; 45923 MW; CAA6102D3B312470 CRC64;
MNKWLCCALL VLLDIIEWTT QETLPPKYLH YDPETGHQLL CDKCAPGTYL
KQHCTVRRKT LCVPCPDHSY TDSWHTSDEC VYCSPVCKEL QSVKQECNRT
HNRVCECEEG RYLEIEFCLK HRSCPPGSGV VQAGTPERNT VCKKCPDGFF
SGETSSKAPC IKHTNCSTFG LLLIQKGNAT HDNVCSGNRE ATQKCGIDVT
LCEEAFFRFA VPTKIIPNWL SVLVDSLPGT KVNAESVERI KRRHSSQEQT
FQLLKLWKHQ NRDQEMVKKI IQDIDLCESS VQRHLGHSNL TTEQLLALME
SLPGKKISPE EIERTRKTCK SSEQLLKLLS LWRIKNGDQD TLKGLMYALK
HLKTSHFPKT VTHSLRKTMR FLHSFTMYRL YQKLFLEMIG NQVQSVKISC
L
UCSC uc007vrk.1; mouse.;

Category

Mouse Tnfrsf11b ELISA Kit