Mouse O3far1 ELISA Kit

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  • Alternative name

    Omega-3 fatty acid receptor 1 ELISA KIT; Omega-3 fatty acid receptor 1O3FAR1 ELISA KIT;

  • Catalog
  • species
  • GeneO3far1
  • Other Species Human O3FAR1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.469ng/ml
  • Intended UseMouse O3far1 ELISA Kit allows for the in vitro quantitative determination of O3far1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse O3far1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse O3far1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse O3far1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse O3far1 can be calculated.

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