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Mouse Olig1 ELISA Kit

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  • Alternative name

    Mouse Oligo1 ELISA Kit;Mouse oligodendrocyte-specific bHLH transcription factor 1 ELISA Kit;Mouse AI836478 ELISA Kit;Mouse AW494459 ELISA Kit;Mouse Bhlhb6 ELISA Kit;Mouse Olg-1 ELISA Kit;Mouse bHLHe21 ELISA Kit;Mouse oligodendrocyte transcription factor 1 ELISA Kit;
  • Catalog
    E062519
  • species
    Mouse
  • GeneOlig1
  • Other Species Human OLIG1 ELISA KitRat Olig1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of OLIG1. No significant cross-reactivity or interference between OLIG1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OLIG1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Olig1 ELISA Kit allows for the in vitro quantitative determination of Olig1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This OLIG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse OLIG1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: OLIG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-OLIG1 antibody and an OLIG1-HRP conjugate. The assay sample and buffer are incubated together with OLIG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OLIG1 concentration since OLIG1 from samples and OLIG1-HRP conjugate compete for the anti-OLIG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by OLIG1 from the sample, fewer sites are left to bind OLIG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OLIG1 concentration in each sample is interpolated from this standard curve.
  • Mouse Oligodendrocyte transcription factor 1 Protein information
  • Uniprot ID OLIG1_MOUSE
  • Uniprot AC Q9JKN5;
  • UniGene Mm.39300;
  • GeneID 50914
  • KEGG mmu:50914;
  • Mouse Oligodendrocyte transcription factor 1 Protein SEQUENCE

  • SEQUENCE 260 AA; 27141 MW; 5EA8A74D9DF0D310 CRC64;

    MYYAISQARV NAAPATMLRP QRPGDVQLGA SLYELVGYRQ PPISSSSSSS

    SSTASLLPKP AREKAEAPLA EPRGPAPESG GARADAKEEQ QQQQLRRKIN

    SRERKRMQDL NLAMDALREV ILPYSAAHCQ GAPGRKLSKI ATLLLARNYI

    LLLGSSLQEL RRALGDGAGP AAPRLLLAGL PLLAAAPGSV LLAPGAVGPP

    ETLRPTKYLS LALDEPPCGQ FSLPAGGAGS PGLCSCAVCK FPHLVPAGLG

    LAAVQAQFSK

  • UCSC uc007zxf.1; mouse.;



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