Mouse Olig1 ELISA Kit

Alternative name
Mouse Oligo1 ELISA Kit;Mouse oligodendrocyte-specific bHLH transcription factor 1 ELISA Kit;Mouse AI836478 ELISA Kit;Mouse AW494459 ELISA Kit;Mouse Bhlhb6 ELISA Kit;Mouse Olg-1 ELISA Kit;Mouse bHLHe21 ELISA Kit;Mouse oligodendrocyte transcription factor 1 ELISA Kit;
Catalog
E062519
species
Mouse
GeneOlig1
SpecificityThis assay has high sensitivity and excellent specificity for detection of OLIG1. No significant cross-reactivity or interference between OLIG1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OLIG1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Olig1 ELISA Kit allows for the in vitro quantitative determination of Olig1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This OLIG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse OLIG1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: OLIG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-OLIG1 antibody and an OLIG1-HRP conjugate. The assay sample and buffer are incubated together with OLIG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OLIG1 concentration since OLIG1 from samples and OLIG1-HRP conjugate compete for the anti-OLIG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by OLIG1 from the sample, fewer sites are left to bind OLIG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OLIG1 concentration in each sample is interpolated from this standard curve.
Mouse Oligodendrocyte transcription factor 1 Protein information
Uniprot ID OLIG1_MOUSE
Uniprot AC Q9JKN5;
UniGene Mm.39300;
GeneID 50914
KEGG mmu:50914;
Mouse Oligodendrocyte transcription factor 1 Protein SEQUENCE
SEQUENCE 260 AA; 27141 MW; 5EA8A74D9DF0D310 CRC64;
MYYAISQARV NAAPATMLRP QRPGDVQLGA SLYELVGYRQ PPISSSSSSS
SSTASLLPKP AREKAEAPLA EPRGPAPESG GARADAKEEQ QQQQLRRKIN
SRERKRMQDL NLAMDALREV ILPYSAAHCQ GAPGRKLSKI ATLLLARNYI
LLLGSSLQEL RRALGDGAGP AAPRLLLAGL PLLAAAPGSV LLAPGAVGPP
ETLRPTKYLS LALDEPPCGQ FSLPAGGAGS PGLCSCAVCK FPHLVPAGLG
LAAVQAQFSK
UCSC uc007zxf.1; mouse.;

Mouse Olig1 ELISA Kit

Mouse Oligo1 ELISA Kit;Mouse oligodendrocyte-specific bHLH transcription factor 1 ELISA Kit;Mouse AI836478 ELISA Kit;Mouse AW494459 ELISA Kit;Mouse Bhlhb6 ELISA Kit;Mouse Olg-1 ELISA Kit;Mouse bHLHe21 ELISA Kit;Mouse oligodendrocyte transcription factor 1 ELISA Kit;

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