Human CNN3 ELISA Kit

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  • Alternative name

    Human calponin, acidic isoform ELISA Kit;Human calponin 3 ELISA Kit;Human calponin-3 ELISA Kit;Human calponin 3, acidic ELISA Kit;Human dJ639P13.2.2 (acidic calponin 3) ELISA Kit;

  • Catalog
    E006227
  • species
    Human
  • GeneCNN3
  • Standard CurveHuman CNN3 ELISA Kit
  • Other Species Mouse Cnn3 ELISA KitBovine CNN3 ELISA KitRat Cnn3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CNN3. No significant cross-reactivity or interference between CNN3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CNN3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CNN3 ELISA Kit allows for the in vitro quantitative determination of CNN3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Intended Uses: This CNN3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CNN3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CNN3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CNN3 antibody and an CNN3-HRP conjugate. The assay sample and buffer are incubated together with CNN3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNN3 concentration since CNN3 from samples and CNN3-HRP conjugate compete for the anti-CNN3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNN3 from the sample, fewer sites are left to bind CNN3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNN3 concentration in each sample is interpolated from this standard curve.
  • Human Calponin-3 Protein information
  • Uniprot ID CNN3_HUMAN
  • Uniprot AC Q15417; B4DFK6; B4DP09; F8WA86; Q6FHA7;
  • UniGene Hs.483454; Hs.736112;
  • GeneID 1266
  • KEGG hsa:1266;
  • Human Calponin-3 Protein SEQUENCE
  • SEQUENCE 329 AA; 36414 MW; F024E169E821A8D8 CRC64;

    MTHFNKGPSY GLSAEVKNKI ASKYDHQAEE DLRNWIEEVT GMSIGPNFQL

    GLKDGIILCE LINKLQPGSV KKVNESSLNW PQLENIGNFI KAIQAYGMKP

    HDIFEANDLF ENGNMTQVQT TLVALAGLAK TKGFHTTIDI GVKYAEKQTR

    RFDEGKLKAG QSVIGLQMGT NKCASQAGMT AYGTRRHLYD PKMQTDKPFD

    QTTISLQMGT NKGASQAGML APGTRRDIYD QKLTLQPVDN STISLQMGTN

    KVASQKGMSV YGLGRQVYDP KYCAAPTEPV IHNGSQGTGT NGSEISDSDY

    QAEYPDEYHG EYQDDYPRDY QYSDQGIDY

  • UCSC uc001dqz.6; human. [Q15417-1];


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