Human CNN1 ELISA Kit

Alternative name
Human basic calponin ELISA Kit;Human calponin H1, smooth muscle ELISA Kit;Human HEL-S-14 ELISA Kit;Human SMCC ELISA Kit;Human Sm-Calp ELISA Kit;Human calponin 1 ELISA Kit;Human calponin-1 ELISA Kit;Human calponin 1, basic, smooth muscle ELISA Kit;Human calponins, basic ELISA Kit;Human epididymis secretory protein Li 14 ELISA Kit;
Catalog
E006219
species
Human
GeneCNN1
SpecificityThis assay has high sensitivity and excellent specificity for detection of CNN1. No significant cross-reactivity or interference between CNN1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CNN1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CNN1 ELISA Kit allows for the in vitro quantitative determination of CNN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalPrinciple of the assay: CNN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CNN1 antibody and an CNN1-HRP conjugate. The assay sample and buffer are incubated together with CNN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNN1 concentration since CNN1 from samples and CNN1-HRP conjugate compete for the anti-CNN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNN1 from the sample, fewer sites are left to bind CNN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNN1 concentration in each sample is interpolated from this standard curve.
Human Calponin-1 Protein information
Uniprot ID CNN1_HUMAN
Uniprot AC P51911; B2R868; B4DUX6; O00638; Q15416; Q8IY93; Q99438;
UniGene Hs.465929;
GeneID 1264
KEGG hsa:1264;
Human Calponin-1 Protein SEQUENCE
SEQUENCE 297 AA; 33170 MW; A949573543BC246C CRC64;
MSSAHFNRGP AYGLSAEVKN KLAQKYDHQR EQELREWIEG VTGRRIGNNF
MDGLKDGIIL CEFINKLQPG SVKKINESTQ NWHQLENIGN FIKAITKYGV
KPHDIFEAND LFENTNHTQV QSTLLALASM AKTKGNKVNV GVKYAEKQER
KFEPGKLREG RNIIGLQMGT NKFASQQGMT AYGTRRHLYD PKLGTDQPLD
QATISLQMGT NKGASQAGMT APGTKRQIFE PGLGMEHCDT LNVSLQMGSN
KGASQRGMTV YGLPRQVYDP KYCLTPEYPE LGEPAHNHHA HNYYNSA

Human CNN1 ELISA Kit

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