Human CNN1 ELISA Kit

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  • Alternative name

    Human basic calponin ELISA Kit;Human calponin H1, smooth muscle ELISA Kit;Human HEL-S-14 ELISA Kit;Human SMCC ELISA Kit;Human Sm-Calp ELISA Kit;Human calponin 1 ELISA Kit;Human calponin-1 ELISA Kit;Human calponin 1, basic, smooth muscle ELISA Kit;Human calponins, basic ELISA Kit;Human epididymis secretory protein Li 14 ELISA Kit;

  • Catalog
    E006219
  • species
    Human
  • GeneCNN1
  • Standard CurveHuman CNN1 ELISA Kit
  • Other Species Mouse Cnn1 ELISA KitBovine CNN1 ELISA KitChicken CNN1 ELISA KitTurkey CNN1 ELISA KitPorcine CNN1 ELISA KitRat Cnn1 ELISA KitSheep CNN1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CNN1. No significant cross-reactivity or interference between CNN1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CNN1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CNN1 ELISA Kit allows for the in vitro quantitative determination of CNN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Principle of the assay: CNN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CNN1 antibody and an CNN1-HRP conjugate. The assay sample and buffer are incubated together with CNN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNN1 concentration since CNN1 from samples and CNN1-HRP conjugate compete for the anti-CNN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNN1 from the sample, fewer sites are left to bind CNN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNN1 concentration in each sample is interpolated from this standard curve.
  • Human Calponin-1 Protein information
  • Uniprot ID CNN1_HUMAN
  • Uniprot AC P51911; B2R868; B4DUX6; O00638; Q15416; Q8IY93; Q99438;
  • UniGene Hs.465929;
  • GeneID 1264
  • KEGG hsa:1264;
  • Human Calponin-1 Protein SEQUENCE
  • SEQUENCE 297 AA; 33170 MW; A949573543BC246C CRC64;

    MSSAHFNRGP AYGLSAEVKN KLAQKYDHQR EQELREWIEG VTGRRIGNNF

    MDGLKDGIIL CEFINKLQPG SVKKINESTQ NWHQLENIGN FIKAITKYGV

    KPHDIFEAND LFENTNHTQV QSTLLALASM AKTKGNKVNV GVKYAEKQER

    KFEPGKLREG RNIIGLQMGT NKFASQQGMT AYGTRRHLYD PKLGTDQPLD

    QATISLQMGT NKGASQAGMT APGTKRQIFE PGLGMEHCDT LNVSLQMGSN

    KGASQRGMTV YGLPRQVYDP KYCLTPEYPE LGEPAHNHHA HNYYNSA



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