Human CAST ELISA Kit

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  • Alternative name

    Human calpain inhibitor ELISA Kit;Human sperm BS-17 component ELISA Kit;Human BS-17 ELISA Kit;Human PLACK ELISA Kit;Human calpastatin ELISA Kit;

  • Catalog
    E006217
  • species
    Human
  • GeneCAST
  • Other Species Mouse Cast ELISA KitBovine CAST ELISA KitPorcine CAST ELISA KitRabbit CAST ELISA KitRat Cast ELISA KitSheep CAST ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human CAST. No significant cross-reactivity or interference between human CAST and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human CAST is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman CAST ELISA Kit allows for the in vitro quantitative determination of CAST , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CAST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CAST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAST is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAST bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Calpastatin Protein information
  • Uniprot ID ICAL_HUMAN
  • Uniprot AC Q9H1Z5;
  • UniGene Hs.436186;
  • GeneID 831
  • Human Calpastatin Protein SEQUENCE
  • SEQUENCE 708 AA; 76573 MW; 2DB0C14B860E89C7 CRC64;

    MNPTETKAIP VSQQMEGPHL PNKKKHKKQA VKTEPEKKSQ STKLSVVHEK

    KSQEGKPKEH TEPKSLPKQA SDTGSNDAHN KKAVSRSAEQ QPSEKSTEPK

    TKPQDMISAG GESVAGITAI SGKPGDKKKE KKSLTPAVPV ESKPDKPSGK

    SGMDAALDDL IDTLGGPEET EEENTTYTGP EVSDPMSSTY IEELGKREVT

    IPPKYRELLA KKEGITGPPA DSSKPIGPDD AIDALSSDFT CGSPTAAGKK

    TEKEESTEVL KAQSAGTVRS AAPPQEKKRK VEKDTMSDQA LEALSASLGT

    RQAEPELDLR SIKEVDEAKA KEEKLEKCGE DDETIPSEYR LKPATDKDGK

    PLLPEPEEKP KPRSESELID ELSEDFDRSE CKEKPSKPTE KTEESKAAAP

    APVSEAVCRT SMCSIQSAPP EPATLKGTVP DDAVEALADS LGKKEADPED

    GKPVMDKVKE KAKEEDREKL GEKEETIPPD YRLEEVKDKD GKPLLPKESK

    EQLPPMSEDF LLDALSEDFS GPQNASSLKF EDAKLAAAIS EVVSQTPAST

    TQAGAPPRDT SQSDKDLDDA LDKLSDSLGQ RQPDPDENKP MEDKVKEKAK

    AEHRDKLGER DDTIPPEYRH LLDDNGQDKP VKPPTKKSED SKKPADDQDP

    IDALSGDLDS CPSTTETSQN TAKDKCKKAA SSSKAPKNGG KAKDSAKTTE

    ETSKPKDD

  • UCSC uc003klt.4; human. [P20810-1];


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