Human CAPNS1 ELISA Kit

Alternative name
Human CSS1 ELISA Kit;Human calcium-activated neutral proteinase small subunit ELISA Kit;Human CANP small subunit ELISA Kit;Human Calcium-dependent protease small subunit ELISA Kit;Human CDPS ELISA Kit;Human calcium-dependent protease small subunit 1 ELISA Kit;Human calpain regulatory subunit ELISA Kit;Human CAPN4 ELISA Kit;Human CAPNS ELISA Kit;Human CALPAIN4 ELISA Kit;Human CANP ELISA Kit;Human CANPS ELISA Kit;Human calpain small subunit 1 ELISA Kit;Human calcium-dependent protease, small subunit ELISA Kit;Human calpain 4, small subunit (30K) ELISA Kit;Human calpain, small polypeptide ELISA Kit;
Catalog
E006208
species
Human
GeneCAPNS1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Calpain, Small Subunit 1 (CAPNS1). No significant cross-reactivity or interference between Calpain, Small Subunit 1 (CAPNS1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.126ng/mL.
Intended UseHuman CAPNS1 ELISA Kit allows for the in vitro quantitative determination of CAPNS1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain, Small Subunit 1 (CAPNS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpain, Small Subunit 1 (CAPNS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calpain, Small Subunit 1 (CAPNS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calpain, Small Subunit 1 (CAPNS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Calpain small subunit 1 Protein information
Uniprot ID CPNS1_HUMAN
Uniprot AC P04632; A8K0P1; Q8WTX3; Q96EW0;
UniGene Hs.515371;
GeneID 826
KEGG hsa:826;
Human Calpain small subunit 1 Protein SEQUENCE
SEQUENCE 268 AA; 28316 MW; 17B87A8E47A90632 CRC64;
MFLVNSFLKG GGGGGGGGGG LGGGLGNVLG GLISGAGGGG GGGGGGGGGG
GGGGGGTAMR ILGGVISAIS EAAAQYNPEP PPPRTHYSNI EANESEEVRQ
FRRLFAQLAG DDMEVSATEL MNILNKVVTR HPDLKTDGFG IDTCRSMVAV
MDSDTTGKLG FEEFKYLWNN IKRWQAIYKQ FDTDRSGTIC SSELPGAFEA
AGFHLNEHLY NMIIRRYSDE SGNMDFDNFI SCLVRLDAMF RAFKSLDKDG
TGQIQVNIQE WLQLTMYS
UCSC uc002odi.2; human.;

Human CAPNS1 ELISA Kit

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