Human CAPNS1 ELISA Kit

Alternative name
Human CSS1 ELISA Kit;Human calcium-activated neutral proteinase small subunit ELISA Kit;Human CANP small subunit ELISA Kit;Human Calcium-dependent protease small subunit ELISA Kit;Human CDPS ELISA Kit;Human calcium-dependent protease small subunit 1 ELISA Kit;Human calpain regulatory subunit ELISA Kit;Human CAPN4 ELISA Kit;Human CAPNS ELISA Kit;Human CALPAIN4 ELISA Kit;Human CANP ELISA Kit;Human CANPS ELISA Kit;Human calpain small subunit 1 ELISA Kit;Human calcium-dependent protease, small subunit ELISA Kit;Human calpain 4, small subunit (30K) ELISA Kit;Human calpain, small polypeptide ELISA Kit;
Catalog
E006208
species
Human
GeneCAPNS1
Standard Curve
Other Species Mouse Capns1 ELISA Kit Bovine CAPNS1 ELISA Kit Porcine CAPNS1 ELISA Kit Rabbit CAPNS1 ELISA Kit Rat Capns1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Calpain, Small Subunit 1 (CAPNS1). No significant cross-reactivity or interference between Calpain, Small Subunit 1 (CAPNS1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.126ng/mL.
Intended UseHuman CAPNS1 ELISA Kit allows for the in vitro quantitative determination of CAPNS1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain, Small Subunit 1 (CAPNS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpain, Small Subunit 1 (CAPNS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calpain, Small Subunit 1 (CAPNS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calpain, Small Subunit 1 (CAPNS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Calpain small subunit 1 Protein information
Uniprot ID CPNS1_HUMAN
Uniprot AC P04632; A8K0P1; Q8WTX3; Q96EW0;
UniGene Hs.515371;
GeneID 826
KEGG hsa:826;
Human Calpain small subunit 1 Protein SEQUENCE
SEQUENCE 268 AA; 28316 MW; 17B87A8E47A90632 CRC64;
MFLVNSFLKG GGGGGGGGGG LGGGLGNVLG GLISGAGGGG GGGGGGGGGG
GGGGGGTAMR ILGGVISAIS EAAAQYNPEP PPPRTHYSNI EANESEEVRQ
FRRLFAQLAG DDMEVSATEL MNILNKVVTR HPDLKTDGFG IDTCRSMVAV
MDSDTTGKLG FEEFKYLWNN IKRWQAIYKQ FDTDRSGTIC SSELPGAFEA
AGFHLNEHLY NMIIRRYSDE SGNMDFDNFI SCLVRLDAMF RAFKSLDKDG
TGQIQVNIQE WLQLTMYS
UCSC uc002odi.2; human.;

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Human CAPNS1 ELISA Kit