Mouse Nup133 ELISA Kit

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  • Alternative name

    Mouse 133 kDa nucleoporin ELISA Kit;Mouse nucleoporin Nup133 ELISA Kit;Mouse mermaid ELISA Kit;Mouse nucleoporin 133 ELISA Kit;Mouse nuclear pore complex protein Nup133 ELISA Kit;

  • Catalog
    E062014
  • species
    Mouse
  • GeneNup133
  • Standard CurveMouse Nup133 ELISA Kit
  • Other Species Human NUP133 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Nup133 ELISA Kit allows for the in vitro quantitative determination of Nup133 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This NUP133 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse NUP133. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: NUP133 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NUP133 antibody and an NUP133-HRP conjugate. The assay sample and buffer are incubated together with NUP133-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NUP133 concentration since NUP133 from samples and NUP133-HRP conjugate compete for the anti-NUP133 antibody binding site. Since the number of sites is limited, as more sites are occupied by NUP133 from the sample, fewer sites are left to bind NUP133-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NUP133 concentration in each sample is interpolated from this standard curve.
  • Mouse Nuclear pore complex protein Nup133 Protein information
  • Uniprot ID NU133_MOUSE
  • Uniprot AC Q8R0G9; E9QLJ8;
  • UniGene Mm.267812;
  • GeneID 234865
  • KEGG mmu:234865;
  • Mouse Nuclear pore complex protein Nup133 Protein SEQUENCE
  • SEQUENCE 1155 AA; 128620 MW; 2B1967D74FA3D683 CRC64;

    MFPSVSSPRT PGPGTRRGPL VGIGPTSTPR ASRRGLSLGS AVNSPVLFSP

    AGRRSSVSSR GTPTRIFPHH SISESVNYDV RVFGSSLPVK IMEALTMAEA

    DEQLSVHVDE GGWACLVCTE KLLIWKIAVS PVTKLSVCKE LQLPPSDFHG

    SADLVALSYA ATSGEVHSVQ AVSVMVATKE GSIRYWPSLA REDTYSDTCV

    DLGGEKMCRF LTAVQGGSFI LSSVGSQLVR LIPESSGKIH QHVLPQGQGM

    LSGIGRRVSS LFGILSPTSD LMLASVLWDR GGSSFYTLTS SNISKWELDD

    SSEKQVHSWD VHRTLKESIT DAVWGSESNY EAIKEGVNIQ YLDLKQNCDG

    LLILAAAWHL GDSPCLVYYS VITVEDNGNQ MSDAVTVEVT QYNPPFQSED

    LIACRLMVPN FSSQMTYLYM ENAVFVCSTG TGKFSLPQEK IVFDTQGDGI

    LGAGSCAGVP ILFSRNSGLV SVTPRENVSL LAEDLEESLT SSVGGRGSES

    MVFETTTKNE TVAHEDKTKL LKAAFLQYCR KDLGRAQIMA DELFSSHTDL

    DSDPELDKAV TQISVDLIDD YPASDPRWAE SVPQEAPGLS NTSLIILHQL

    EDKMKAHCLL VDFLHQVGLF RRLSSYPIRG TPMSTRLLLC EHAEKLSAAI

    TLKNHHSRLP DLVNSAILLA LNKRECEVPN SLTPADVFFR EVSQVDTICE

    CLLEHEEQVL REVALVSQEW AEVAIDVNTV LKDMLQAATH YRLNKSSMYS

    QEEVLGKEPE YVPWTATSGP SGIRTAVMRQ HGIILKMVYP QADSKLRNVV

    MEQLVALIDC FLDSYVSQLK SLEKSSDQER YSSLEVEYLQ KRSELLSPLL

    TLGQYPWAAS LAEKYCDFDI LVQMCEQTDN QARLQRYMTQ FADQNFSDFL

    FRWYLEKGKR GKLLSQPISQ HGQLANFLQA HEHLSWLHEI NSQELEKAHT

    TLLGLANMET RYFAKKKTLL GLSKLAALAS DISEDRLQEK IEAMAEQERF

    LLHQETLPEQ LLTERQLSLS AMPVLTAPQL ISLYICDENR RANEYDFKKA

    LDLLEYIDEE EDVSIDDLKL EILCRALQRD DWSGSDGKDD PIEVSKDSVF

    VKILQKLIKD GIQLSEYLPE VTDLLRAEQL GSLKSNSYFE FVLKANYEYY

    VQGQM

  • UCSC uc009nws.2; mouse.;


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