Mouse Nup133 ELISA Kit

Alternative name
Mouse 133 kDa nucleoporin ELISA Kit;Mouse nucleoporin Nup133 ELISA Kit;Mouse mermaid ELISA Kit;Mouse nucleoporin 133 ELISA Kit;Mouse nuclear pore complex protein Nup133 ELISA Kit;
Catalog
E062014
species
Mouse
GeneNup133
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Nup133 ELISA Kit allows for the in vitro quantitative determination of Nup133 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This NUP133 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse NUP133. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: NUP133 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NUP133 antibody and an NUP133-HRP conjugate. The assay sample and buffer are incubated together with NUP133-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NUP133 concentration since NUP133 from samples and NUP133-HRP conjugate compete for the anti-NUP133 antibody binding site. Since the number of sites is limited, as more sites are occupied by NUP133 from the sample, fewer sites are left to bind NUP133-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NUP133 concentration in each sample is interpolated from this standard curve.
Mouse Nuclear pore complex protein Nup133 Protein information
Uniprot ID NU133_MOUSE
Uniprot AC Q8R0G9; E9QLJ8;
UniGene Mm.267812;
GeneID 234865
KEGG mmu:234865;
Mouse Nuclear pore complex protein Nup133 Protein SEQUENCE
SEQUENCE 1155 AA; 128620 MW; 2B1967D74FA3D683 CRC64;
MFPSVSSPRT PGPGTRRGPL VGIGPTSTPR ASRRGLSLGS AVNSPVLFSP
AGRRSSVSSR GTPTRIFPHH SISESVNYDV RVFGSSLPVK IMEALTMAEA
DEQLSVHVDE GGWACLVCTE KLLIWKIAVS PVTKLSVCKE LQLPPSDFHG
SADLVALSYA ATSGEVHSVQ AVSVMVATKE GSIRYWPSLA REDTYSDTCV
DLGGEKMCRF LTAVQGGSFI LSSVGSQLVR LIPESSGKIH QHVLPQGQGM
LSGIGRRVSS LFGILSPTSD LMLASVLWDR GGSSFYTLTS SNISKWELDD
SSEKQVHSWD VHRTLKESIT DAVWGSESNY EAIKEGVNIQ YLDLKQNCDG
LLILAAAWHL GDSPCLVYYS VITVEDNGNQ MSDAVTVEVT QYNPPFQSED
LIACRLMVPN FSSQMTYLYM ENAVFVCSTG TGKFSLPQEK IVFDTQGDGI
LGAGSCAGVP ILFSRNSGLV SVTPRENVSL LAEDLEESLT SSVGGRGSES
MVFETTTKNE TVAHEDKTKL LKAAFLQYCR KDLGRAQIMA DELFSSHTDL
DSDPELDKAV TQISVDLIDD YPASDPRWAE SVPQEAPGLS NTSLIILHQL
EDKMKAHCLL VDFLHQVGLF RRLSSYPIRG TPMSTRLLLC EHAEKLSAAI
TLKNHHSRLP DLVNSAILLA LNKRECEVPN SLTPADVFFR EVSQVDTICE
CLLEHEEQVL REVALVSQEW AEVAIDVNTV LKDMLQAATH YRLNKSSMYS
QEEVLGKEPE YVPWTATSGP SGIRTAVMRQ HGIILKMVYP QADSKLRNVV
MEQLVALIDC FLDSYVSQLK SLEKSSDQER YSSLEVEYLQ KRSELLSPLL
TLGQYPWAAS LAEKYCDFDI LVQMCEQTDN QARLQRYMTQ FADQNFSDFL
FRWYLEKGKR GKLLSQPISQ HGQLANFLQA HEHLSWLHEI NSQELEKAHT
TLLGLANMET RYFAKKKTLL GLSKLAALAS DISEDRLQEK IEAMAEQERF
LLHQETLPEQ LLTERQLSLS AMPVLTAPQL ISLYICDENR RANEYDFKKA
LDLLEYIDEE EDVSIDDLKL EILCRALQRD DWSGSDGKDD PIEVSKDSVF
VKILQKLIKD GIQLSEYLPE VTDLLRAEQL GSLKSNSYFE FVLKANYEYY
VQGQM
UCSC uc009nws.2; mouse.;

Category

Mouse Nup133 ELISA Kit

Mouse 133 kDa nucleoporin ELISA Kit;Mouse nucleoporin Nup133 ELISA Kit;Mouse mermaid ELISA Kit;Mouse nucleoporin 133 ELISA Kit;Mouse nuclear pore complex protein Nup133 ELISA Kit;