Mouse Nrf1 ELISA Kit

Alternative name
Mouse NRF-1 ELISA Kit;Mouse alpha palindromic-binding protein ELISA Kit;Mouse alpha-pal ELISA Kit;Mouse C87038 ELISA Kit;Mouse D6Ertd415e ELISA Kit;Mouse nuclear respiratory factor 1 ELISA Kit;
Catalog
E061972
species
Mouse
GeneNrf1
Other Species Human NRF1 ELISA Kit Rat Nrf1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse NRF1. No significant cross-reactivity or interference between mouse NRF1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of mouse NRF1 is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseMouse Nrf1 ELISA Kit allows for the in vitro quantitative determination of Nrf1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NRF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NRF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Nuclear respiratory factor 1 Protein information
Uniprot ID NRF1_MOUSE
Uniprot AC Q9WU00; Q9CXE4;
UniGene Mm.259258;
GeneID 18181
KEGG mmu:18181;
Mouse Nuclear respiratory factor 1 Protein SEQUENCE
SEQUENCE 503 AA; 53571 MW; 6C3D6B8C83C3B227 CRC64;
MEEHGVTQTE HMATIEAHAV AQQVQQVHVA TYTEHSMLSA DEDSPSSPED
TSYDDSDILN STAADEVTAH LAAAGPVGMA AAAAVATGKK RKRPHVFESN
PSIRKRQQTR LLRKLRATLD EYTTRVGQQA IVLCISPSKP NPVFKVFGAA
PLENVVRKYK SMILEDLESA LAEHAPAPQE VNSELPPLTI DGIPVSVDKM
TQAQLRAFIP EMLKYSTGRG KPGWGKESCK PIWWPEDIPW ANVRSDVRTE
EQKQRVSWTQ ALRTIVKNCY KQHGREDLLY AFEDQQTQTQ ATTTHSIAHL
VPSQTVVQTF SNPDGTVSLI QVGTGATVAT LADASELPTT VTVAQVNYSA
VADGEVEQNW ATLQGGEMTI QTTQASEATQ AVASLAEAAV AASQEMQQGA
TVTMALNSEA AAHAVATLAE ATLQGGGQIV LSGETAAAVG ALTGVQDANG
LVQIPVSMYQ TVVTSLAQGN GPVQVAMAPV TTRISDSAVT MDGQAVEVVT
LEQ
UCSC uc009bev.1; mouse. [Q9WU00-1];

Category

Mouse Nrf1 ELISA Kit

Mouse NRF-1 ELISA Kit;Mouse alpha palindromic-binding protein ELISA Kit;Mouse alpha-pal ELISA Kit;Mouse C87038 ELISA Kit;Mouse D6Ertd415e ELISA Kit;Mouse nuclear respiratory factor 1 ELISA Kit;