Human CAPN3 ELISA Kit

Alternative name
Human Calcium-activated neutral proteinase 3 ELISA Kit;Human CANP 3 ELISA Kit;Human Calpain L3 ELISA Kit;Human Calpain p94 ELISA Kit;Human Muscle-specific calcium-activated neutral protease 3 ELISA Kit;Human new calpain 1 ELISA Kit;Human nCL-1 ELISA Kit;Human CANP3 ELISA Kit;Human CANPL3 ELISA Kit;Human NCL1 ELISA Kit;Human LGMD2 ELISA Kit;Human LGMD2A ELISA Kit;Human p94 ELISA Kit;Human calpain 3 ELISA Kit;Human calpain-3 ELISA Kit;Human calpain p94, large [catalytic] subunit ELISA Kit;Human calpain, large polypeptide L3 ELISA Kit;Human muscle-specific calcium-activated neutral protease 3 large subunit ELISA Kit;
Catalog
E006189
species
Human
GeneCAPN3
Standard Curve
Other Species Mouse Capn3 ELISA Kit Bovine CAPN3 ELISA Kit Chicken CAPN3 ELISA Kit Monkey CAPN3 ELISA Kit Porcine CAPN3 ELISA Kit Rat Capn3 ELISA Kit Sheep CAPN3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Calpain 3 (CAPN3). No significant cross-reactivity or interference between Calpain 3 (CAPN3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.125ng/mL.
Intended UseHuman CAPN3 ELISA Kit allows for the in vitro quantitative determination of CAPN3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain 3 (CAPN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpain 3 (CAPN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calpain 3 (CAPN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calpain 3 (CAPN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Calpain-3 Protein information
Uniprot ID CAN3_HUMAN
Uniprot AC P20807; A6H8K6; Q7L4R0; Q9BQC8; Q9BTU4; Q9Y5S6; Q9Y5S7;
UniGene Hs.143261;
GeneID 825
KEGG hsa:825;
Human Calpain-3 Protein SEQUENCE
SEQUENCE 821 AA; 94254 MW; BC608E8B67AA2741 CRC64;
MPTVISASVA PRTAAEPRSP GPVPHPAQSK ATEAGGGNPS GIYSAIISRN
FPIIGVKEKT FEQLHKKCLE KKVLYVDPEF PPDETSLFYS QKFPIQFVWK
RPPEICENPR FIIDGANRTD ICQGELGDCW FLAAIACLTL NQHLLFRVIP
HDQSFIENYA GIFHFQFWRY GEWVDVVIDD CLPTYNNQLV FTKSNHRNEF
WSALLEKAYA KLHGSYEALK GGNTTEAMED FTGGVAEFFE IRDAPSDMYK
IMKKAIERGS LMGCSIDDGT NMTYGTSPSG LNMGELIARM VRNMDNSLLQ
DSDLDPRGSD ERPTRTIIPV QYETRMACGL VRGHAYSVTG LDEVPFKGEK
VKLVRLRNPW GQVEWNGSWS DRWKDWSFVD KDEKARLQHQ VTEDGEFWMS
YEDFIYHFTK LEICNLTADA LQSDKLQTWT VSVNEGRWVR GCSAGGCRNF
PDTFWTNPQY RLKLLEEDDD PDDSEVICSF LVALMQKNRR KDRKLGASLF
TIGFAIYEVP KEMHGNKQHL QKDFFLYNAS KARSKTYINM REVSQRFRLP
PSEYVIVPST YEPHQEGEFI LRVFSEKRNL SEEVENTISV DRPVKKKKTK
PIIFVSDRAN SNKELGVDQE SEEGKGKTSP DKQKQSPQPQ PGSSDQESEE
QQQFRNIFKQ IAGDDMEICA DELKKVLNTV VNKHKDLKTH GFTLESCRSM
IALMDTDGSG KLNLQEFHHL WNKIKAWQKI FKHYDTDQSG TINSYEMRNA
VNDAGFHLNN QLYDIITMRY ADKHMNIDFD SFICCFVRLE GMFRAFHAFD
KDGDGIIKLN VLEWLQLTMY A
UCSC uc001zpn.2; human. [P20807-1];

Related products with Human CAPN3 ELISA Kit

Category

Human CAPN3 ELISA Kit