Mouse Nfkb1 ELISA Kit

Alternative name
Mouse DNA-binding factor KBF1 ELISA Kit;Mouse EBP-1 ELISA Kit;Mouse NF-kappa-B1 p84/NF-kappa-B1 p98 ELISA Kit;Mouse Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA Kit;Mouse NF-KB1 ELISA Kit;Mouse NF-kappaB ELISA Kit;Mouse NF-kappaB1 ELISA Kit;Mouse p105 ELISA Kit;Mouse p50 ELISA Kit;Mouse p50/p105 ELISA Kit;Mouse nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 ELISA Kit;Mouse nuclear factor NF-kappa-B p105 subunit ELISA Kit;Mouse NF kappaB1 ELISA Kit;Mouse NF-kappaB p50 ELISA Kit;Mouse nuclear factor kappaB p50 ELISA Kit;Mouse nuclear factor of kappa light chain gene enhancer in B-cells 1, p105 ELISA Kit;Mouse p50 subunit of NF kappaB ELISA Kit;
Catalog
E061884
species
Mouse
GeneNfkb1
Other Species Human NFKB1/p105 ELISA Kit Human NFKB1 ELISA Kit Mouse NFKB1/p105 ELISA Kit Canine NFKB1 ELISA Kit Chicken NFKB1 ELISA Kit Rat Nfkb1 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseMouse Nfkb1 ELISA Kit allows for the in vitro quantitative determination of Nfkb1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Nfkb1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Nfkb1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Nfkb1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Nfkb1 can be calculated.
Mouse Nuclear factor NF-kappa-B p50 subunit Protein information
Uniprot ID NFKB1_MOUSE
Uniprot AC P25799; B2RRQ6; Q3TZE8; Q3V2V6; Q6TDG8; Q75ZL1; Q80Y21; Q8C712;
UniGene Mm.256765;
GeneID 18033
KEGG mmu:18033;
Mouse Nuclear factor NF-kappa-B p50 subunit Protein SEQUENCE
SEQUENCE 971 AA; 105615 MW; 91EA9C595E375C30 CRC64;
MADDDPYGTG QMFHLNTALT HSIFNAELYS PEIPLSTDGP YLQILEQPKQ
RGFRFRYVCE GPSHGGLPGA SSEKNKKSYP QVKICNYVGP AKVIVQLVTN
GKNIHLHAHS LVGKHCEDGV CTVTAGPKDM VVGFANLGIL HVTKKKVFET
LEARMTEACI RGYNPGLLVH SDLAYLQAEG GGDRQLTDRE KEIIRQAAVQ
QTKEMDLSVV RLMFTAFLPD STGSFTRRLE PVVSDAIYDS KAPNASNLKI
VRMDRTAGCV TGGEEIYLLC DKVQKDDIQI RFYEEEENGG VWEGFGDFSP
TDVHRQFAIV FKTPKYKDVN ITKPASVFVQ LRRKSDLETS EPKPFLYYPE
IKDKEEVQRK RQKLMPNFSD SFGGGSGAGA GGGGMFGSGG GGGSTGSPGP
GYGYSNYGFP PYGGITFHPG VTKSNAGVTH GTINTKFKNG PKDCAKSDDE
ESLTLPEKET EGEGPSLPMA CTKTEPIALA STMEDKEQDM GFQDNLFLEK
ALQLARRHAN ALFDYAVTGD VKMLLAVQRH LTAVQDENGD SVLHLAIIHL
HAQLVRDLLE VTSGLISDDI INMRNDLYQT PLHLAVITKQ EDVVEDLLRV
GADLSLLDRW GNSVLHLAAK EGHDRILSIL LKSRKAAPLI DHPNGEGLNA
IHIAVMSNSL PCLLLLVAAG AEVNAQEQKS GRTALHLAVE YDNISLAGCL
LLEGDAHVDS TTYDGTTPLH IAAGRGSTRL AALLKAAGAD PLVENFEPLY
DLDDSWEKAG EDEGVVPGTT PLDMAANWQV FDILNGKPYE PVFTSDDILP
QGDMKQLTED TRLQLCKLLE IPDPDKNWAT LAQKLGLGIL NNAFRLSPAP
SKTLMDNYEV SGGTIKELME ALQQMGYTEA IEVIQAAFRT PATTASSPVT
TAQVHCLPLS SSSTRQHIDE LRDSDSVCDS GVETSFRKLS FTESLTGDSP
LLSLNKMPHG YGQEGPIEGK I
UCSC uc012cyf.1; mouse. [P25799-6];

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Mouse Nfkb1 ELISA Kit