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Alternative name
Nuclear factor erythroid 2-related factor 2 ELISA KIT; HEBP1 ELISA KIT; Nuclear factor, erythroid derived 2, like 2NFE2L2 ELISA KIT; NRF2 ELISA KIT; NF-E2-related factor 2 ELISA KIT; NFE2-related factor 2 ELISA KIT
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Catalog
E061868
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species
Mouse
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GeneNrf2
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Other Species
Human Nrf2 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of NFE2R2. No significant cross-reactivity or interference between NFE2R2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NFE2R2 and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseMouse Nrf2 ELISA Kit allows for the in vitro quantitative determination of Nrf2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: This NFE2R2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse NFE2R2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||NFE2R2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for NFE2R2. Standards or samples are then added to the microtiter plate wells and NFE2R2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of NFE2R2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for NFE2R2 are added to each well to "sandwich" the NFE2R2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain NFE2R2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFE2R2 concentration in each sample is interpolated from this standard curve.
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