Sensitivity Intended Uses For detection of NT-4 in Mouse serum, plasma, urine, tissue samples or cell c
Intended UseMouse NT-4 ELISA Kit allows for the in vitro quantitative determination of NT-4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-NT-4 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-NT-4 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NT-4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NT-4 can be calculated.
Background: Neurotrophins act as survival and differentiation factors in the nervous system and have been detected in the developing rodent testis. Three additional structurally homologous neurotrophic factors have been identified. These include brainderived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). Neurotrophin-4 (NT-4), is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. Mammalian NT4 had many unusual features compared to the previously identified neurotrophins and was less conserved evolutionarily than the others; however, mammalian NT4 displayed bioactivity and TRK receptor specificity similar to that of Xenopus NT4.