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Alternative name
Nitric oxide synthase, inducible ELISA KIT; Hepatocyte NOS ELISA KIT; HEP-NOS ELISA KIT; Inducible NO synthase ELISA KIT; Inducible NOS ELISA KIT; iNOS ELISA KIT; NOS type II ELISA KIT; Peptidyl-cysteine S-nitrosylase NOS2NOS2 ELISA KIT; NOS2A ELISA KIT; HEP-NOS ELISA KIT; Inducible NOS ELISA KIT; iNOS ELISA KIT
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Catalog
E061817
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species
Mouse
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GeneNOS2/iNOS
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse NOS2/iNOS. No significant cross-reactivity or interference between Mouse NOS2/iNOS and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse NOS2/iNOS and all the analogues, therefore, cross reaction may still exist.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.188ng/mL
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Intended UseMouse NOS2/iNOS ELISA Kit allows for the in vitro quantitative determination of NOS2/iNOS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NOS2/iNOS in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS2/iNOS. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse NOS2/iNOS. You can calculate the concentration of Mouse NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.
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