Mouse Nampt ELISA Kit

Alternative name
Mouse NAmPRTase ELISA Kit;Mouse Nampt ELISA Kit;Mouse pre-B-cell colony-enhancing factor 1 homolog ELISA Kit;Mouse Pbef ELISA Kit;Mouse Visfatin ELISA Kit;Mouse Pbef1 ELISA Kit;Mouse 1110035O14Rik ELISA Kit;Mouse AI314458 ELISA Kit;Mouse AI480535 ELISA Kit;Mouse nicotinamide phosphoribosyltransferase ELISA Kit;
Catalog
E061653
species
Mouse
GeneNampt
Other Species Human NAMPT ELISA Kit Porcine NAMPT ELISA Kit Rat Nampt ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse NAMPT. No significant cross-reactivity or interference between Mouse NAMPT and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.078 ng/ml
Intended UseMouse Nampt ELISA Kit allows for the in vitro quantitative determination of Nampt , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Visfatin , also know as Nampt or PBEF, is a nicotinamide phosphoribosyltransferase (Nampt) enzyme that catalyzes first step in the biosynthesis of NAD from nicotinamide. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis. Visfatin was originally cloned as a putative cytokine shown to enhance the maturation of B cell precursors in the presence of Interleukin (IL)-7 and stem cell factor, it was therefore named "pre-B cell colony-enhancing factor" (PBEF). When the gene encoding the bacterial nicotinamide phosphoribosyltransferase (nadV) was first isolated in Haemophilus ducreyi, it was found to exhibit significant homology to the mammalian PBEF gene. Rongvaux et al. demonstrated genetically that the human PBEF gene conferred Nampt enzymatic activity and NAD-independent growth to bacteria lacking nadV. Revollo et al. determined biochemically that the human PBEF gene product encodes a Nampt enzyme, capable of modulating intracellular NAD levels. Others have since confirmed these findings. More recently, several groups have reported the crystal structure of Visfatin and they all show that this protein is a dimeric type II phosphoribosyltransferase enzyme involved in NAD biosynthesis. Although the original cytokine function of PBEF has not been confirmed to date, others have since reported or suggested a cytokine-like function for this protein. Particularly, Nampt/PBEF was recently re-identified as a "new visceral fat-derived hormone" named visfatin. It is reported that visfatin is enriched in the visceral fat of both humans and mice and that its plasma levels increase during the development of obesity. Strikingly, visfatin is reported to exert insulin-mimetic effects in cultured cells and to lower plasma glucose levels in mice by binding to and activating the insulin receptor (10). However, the physiological relevance of visfatin is still in question because its plasma concentration is 40 to 100-fold lower than that of insulin despite having similar receptor-binding affinity. Additionally, the ability of visfatin to bind and activate the insulin-receptor has yet to be confirmed by other groups. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Visfatin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Visfatin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Visfatin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Visfatin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Nicotinamide phosphoribosyltransferase Protein information
Uniprot ID NAMPT_MOUSE
Uniprot AC Q99KQ4; Q8C3B5; Q9JKM0;
UniGene Mm.202727;
GeneID 59027
KEGG mmu:59027;
Mouse Nicotinamide phosphoribosyltransferase Protein SEQUENCE
SEQUENCE 491 AA; 55447 MW; D3E522A1841297E6 CRC64;
MNAAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKVR
KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKEVYREH FQDDVFNERG
WNYILEKYDG HLPIEVKAVP EGSVIPRGNV LFTVENTDPE CYWLTNWIET
ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS
QETAGIGASA HLVNFKGTDT VAGIALIKKY YGTKDPVPGY SVPAAEHSTI
TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV
SRSTEAPLII RPDSGNPLDT VLKVLDILGK KFPVTENSKG YKLLPPYLRV
IQGDGVDINT LQEIVEGMKQ KKWSIENVSF GSGGALLQKL TRDLLNCSFK
CSYVVTNGLG VNVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL
EEYGHDLLHT VFKNGKVTKS YSFDEVRKNA QLNIEQDVAP H
UCSC uc007nif.2; mouse.;

Category

Mouse Nampt ELISA Kit