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Mouse Nnt ELISA Kit

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  • Alternative name

    Mouse Nicotinamide nucleotide transhydrogenase ELISA Kit;Mouse Pyridine nucleotide transhydrogenase ELISA Kit;
  • Catalog
    E061652
  • species
    Mouse
  • GeneNnt
  • Other Species Human NNT ELISA KitBovine NNT ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse NNT. No significant cross-reactivity or interference between mouse NNT and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of mouse NNT is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseMouse Nnt ELISA Kit allows for the in vitro quantitative determination of Nnt , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NNT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NNT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NNT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NNT bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Mouse NAD(P) transhydrogenase, mitochondrial Protein information
  • Uniprot ID NNTM_MOUSE
  • Uniprot AC Q61941; Q9JK70;
  • UniGene Mm.132584; Mm.486065;
  • Mouse NAD(P) transhydrogenase, mitochondrial Protein SEQUENCE

  • SEQUENCE 1086 AA; 113838 MW; A023285169834D07 CRC64;

    MAHLLKTVVA GCSCPFLSNL GSSKVLPGKR DFVRTLRTHQ ALWCKSPVKP

    GIPYKQLTVG VPKEIFQNEK RVALSPAGVQ ALVKQGFNVV VESGAGEASK

    FPDDLYRAAG AQIQGMKEVL ASDLVVKVRA PMVNPTLGAH EADFLKPSGT

    LISFIYPAQN PDLLNKLSER KTTVLAMDQV PRVTIAQGYD ALSSMANISG

    YKAVVLAANH FGRFFTGQIT AAGKVPPAKI LIVGGGVAGL ASAGAAKSMG

    AVVRGFDTRA AALEQFKSLG AEPLEVDLKE SGEGQGGYAK EMSKEFIEAE

    MKLFAQQCKE VDILISTALI PGKKAPVLFS KEMIESMKEG SVVVDLAAEA

    GGNFETTKPG ELYVHKGITH IGYTDLPSRM ATQASTLYSN NITKLLKAIS

    PDKDNFHFEV KDDFDFGTMS HVIRGTVVMK DGKVIFPAPT PKNIPEEAPV

    KPKTVAELEA EKAGTVSMYT KTLTTASVYS AGLTGMLGLG IVAPNVAFSQ

    MVTTFGLAGI IGYHTVWGVT PALHSPLMSV TNAISGLTAV GGLALMGGHF

    YPSTTSQSLA ALATFISSVN IAGGFLVTQR MLDMFKRPTD PPEYNYLYLL

    PGGTFVGGYL AALYGGYNIE EIMYLGSGLC CVGALGGLST QGTARLGNAL

    GMIGVAGGLA ATLGGLKPDP QLLAQMSGAM AMGGTIGLTI AKRIQISDLP

    QLVAAFHSLV GLAAVLTCMA EYIVEYPHFA MDATSNFTKI VAYLGTYIGG

    VTFSGSLVAY GKLQGILKSA PLLLPGRHAL NAGLLAASVG GIIPFMADPS

    FTTGITCLGS VSGLSTLMGV TLTAAIGGAD MPVVITVLNS YSGWALCAEG

    FLLNNNLLTI VGALIGSSGA ILSYIMCVAM NRSLANVILG GYGTTSTAGG

    KPMEISGTHT EINLDNAVEM IREANSIVIT PGYGLCAAKA QYPIADLVKM

    LTEQGKKVRF GIHPVAGRMP GQLNVLLAEA GVPYDIVLEM DEINSDFPDT

    DLVLVIGAND TVNSAAQEDP NSIIAGMPVL EVWKSKQVIV MKRSLGVGYA

    AVDNPIFYKP NTAMLLGDAK KTCDALQAKV RESYQK



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