Intended UseMouse Npy ELISA Kit allows for the in vitro quantitative determination of Npy , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalIntroduction: Neuropeptide Y is a 36-amino acid peptide that is abundant throughout the mammalian nervous system. It belongs to the same family of carboxyl-terminally amidated peptides as pancreatic polypeptide and peptide YY. The gene encoding the rat neuropeptide Y precursor spans 7.2 kilobase pairs and contains four exons. The exon organization is identical to the pancreatic polypeptide gene, although the amino acid sequences of the neuropeptide Y and pancreatic polypeptide precursors differ extensively. The predicted amino acid sequence of mature rat neuropeptide Y is identical to the human sequence. The sequence of the 30-amino acid carboxyl-terminal peptide of preproneuropeptide Y is highly conserved, suggests that it is functionally important. Two neuropeptide Y alleles are different at nine positions in 2.5 kilobase pairs at the 5' portion of the gene. One nucleotide substitution close to the gene promoter may influence the regulation of expression. The neuropeptide Y is synthesized throughout the rat brain, peripheral organs, heart and spleen.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NP-Y. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NP-Y and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NP-Y, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NP-Y in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse C-flanking peptide of NPY Protein information