Nitric oxide synthase, brain ELISA KIT; Constitutive NOS ELISA KIT; NC-NOS ELISA KIT; NOS type I ELISA KIT; Neuronal NOS ELISA KIT; N-NOS ELISA KIT; nNOS ELISA KIT; Peptidyl-cysteine S-nitrosylase NOS1 ELISA KIT; bNOSNOS1 ELISA KIT; N-NOS ELISA KIT; nNOS ELISA KIT
Catalog
E061445
species
Mouse
GenenNOS
SpecificityThis assay has high sensitivity and excellent specificity for detection of nNOS. No significant cross-reactivity or interference between nNOS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between nNOS and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseMouse nNOS ELISA Kit allows for the in vitro quantitative determination of nNOS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses||This nNOS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse nNOS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: nNOS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-nNOS antibody and an nNOS-HRP conjugate. The assay sample and buffer are incubated together with nNOS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the nNOS concentration since nNOS from samples and nNOS-HRP conjugate compete for the anti-nNOS antibody binding site. Since the number of sites is limited, as more sites are occupied by nNOS from the sample, fewer sites are left to bind nNOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The nNOS concentration in each sample is interpolated from this standard curve.
No manual or datasheet available for this product.