Mouse Nefh ELISA Kit

Alternative name
Mouse NF-H ELISA Kit;Mouse 200 kDa neurofilament protein ELISA Kit;Mouse neurofilament triplet H protein ELISA Kit;Mouse Kiaa0845 ELISA Kit;Mouse Nfh ELISA Kit;Mouse NF200 ELISA Kit;Mouse mKIAA0845 ELISA Kit;Mouse neurofilament, heavy polypeptide ELISA Kit;Mouse neurofilament heavy polypeptide ELISA Kit;Mouse neurofilament 200kDa ELISA Kit;
Catalog
E061356
species
Mouse
GeneNefh
Standard Curve
Other Species Human NEFH ELISA Kit Porcine NEFH ELISA Kit Rat Nefh ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of NEFH. No significant cross-reactivity or interference between NEFH and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity5.9pg/mL.
Intended UseMouse Nefh ELISA Kit allows for the in vitro quantitative determination of Nefh , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NEFH in mouse serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEFH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NEFH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NEFH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NEFH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Neurofilament heavy polypeptide Protein information
Uniprot ID NFH_MOUSE
Uniprot AC P19246; A1E2H9; Q5SVF6; Q61959;
UniGene Mm.298283;
GeneID 380684
KEGG mmu:380684;
Mouse Neurofilament heavy polypeptide Protein SEQUENCE
SEQUENCE 1090 AA; 116994 MW; B2A7D7D36FF2F448 CRC64;
MMSFGSADAL LGAPFAPLHG GGSLHYSLSR KAGPGGTRSA AGSSSGFHSW
ARTSVSSVSA SPSRFRGAAS STDSLDTLSN GPEGCVVAAV AARSEKEQLQ
ALNDRFAGYI DKVRQLEAHN RSLEGEAAAL RQQQAGRAAM GELYEREVRE
MRGAVLRLGA ARGQLRLEQE HLLEDIAHVR QRLDEEARQR EEAEAAARAL
ARFAQEAEAA RVELQKKAQA LQEECGYLRR HHQEEVGELL GQIQGCGAAQ
AQAQAEARDA LKCDVTSALR EIRAQLEGHA VQSTLQSEEW FRVRLDRLSE
AAKVNTDAMR SAQEEITEYR RQLQARTTEL EALKSTKESL ERQRSELEDR
HQADIASYQD AIQQLDSELR NTKWEMAAQL REYQDLLNVK MALDIEIAAY
RKLLEGEECR IGFGPSPFSL TEGLPKIPSI STHIKVKSEE MIKVVEKSEK
ETVIVEGQTE EIRVTEGVTE EEDKEAQGQE GEEAEEGEEK EEEEGAAATS
PPAEEAASPE KETKSRVKEE AKSPGEAKSP GEAKSPAEAK SPGEAKSPGE
AKSPGEAKSP AEPKSPAEPK SPAEAKSPAE PKSPATVKSP GEAKSPSEAK
SPAEAKSPAE AKSPAEAKSP AEAKSPAEAK SPAEAKSPAT VKSPGEAKSP
SEAKSPAEAK SPAEAKSPAE AKSPAEVKSP GEAKSPAEPK SPAEAKSPAE
VKSPAEAKSP AEVKSPGEAK SPAAVKSPAE AKSPAAVKSP GEAKSPGEAK
SPAEAKSPAE AKSPIEVKSP EKAKTPVKEG AKSPAEAKSP EKAKSPVKED
IKPPAEAKSP EKAKSPVKEG AKPPEKAKPL DVKSPEAQTP VQEEAKHPTD
IRPPEQVKSP AKEKAKSPEK EEAKTSEKVA PKKEEVKSPV KEEVKAKEPP
KKVEEEKTLP TPKTEAKESK KDEAPKEAPK PKVEEKKETP TEKPKDSTAE
AKKEEAGEKK KAVASEEETP AKLGVKEEAK PKEKTETTKT EAEDTKAKEP
SKPTETEKPK KEEMPAAPEK KDTKEEKTTE SRKPEEKPKM EAKVKEDDKS
LSKEPSKPKT EKAEKSSSTD QKESQPPEKT TEDKATKGEK
UCSC uc007hvm.1; mouse.;

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Mouse Nefh ELISA Kit