Mouse Nefh ELISA Kit

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  • Alternative name

    Mouse NF-H ELISA Kit;Mouse 200 kDa neurofilament protein ELISA Kit;Mouse neurofilament triplet H protein ELISA Kit;Mouse Kiaa0845 ELISA Kit;Mouse Nfh ELISA Kit;Mouse NF200 ELISA Kit;Mouse mKIAA0845 ELISA Kit;Mouse neurofilament, heavy polypeptide ELISA Kit;Mouse neurofilament heavy polypeptide ELISA Kit;Mouse neurofilament 200kDa ELISA Kit;

  • Catalog
    E061356
  • species
    Mouse
  • GeneNefh
  • Standard CurveMouse Nefh ELISA Kit
  • Other Species Human NEFH ELISA KitPorcine NEFH ELISA KitRat Nefh ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of NEFH. No significant cross-reactivity or interference between NEFH and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity5.9pg/mL.
  • Intended UseMouse Nefh ELISA Kit allows for the in vitro quantitative determination of Nefh , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NEFH in mouse serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEFH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NEFH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NEFH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NEFH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Neurofilament heavy polypeptide Protein information
  • Uniprot ID NFH_MOUSE
  • Uniprot AC P19246; A1E2H9; Q5SVF6; Q61959;
  • UniGene Mm.298283;
  • GeneID 380684
  • KEGG mmu:380684;
  • Mouse Neurofilament heavy polypeptide Protein SEQUENCE
  • SEQUENCE 1090 AA; 116994 MW; B2A7D7D36FF2F448 CRC64;

    MMSFGSADAL LGAPFAPLHG GGSLHYSLSR KAGPGGTRSA AGSSSGFHSW

    ARTSVSSVSA SPSRFRGAAS STDSLDTLSN GPEGCVVAAV AARSEKEQLQ

    ALNDRFAGYI DKVRQLEAHN RSLEGEAAAL RQQQAGRAAM GELYEREVRE

    MRGAVLRLGA ARGQLRLEQE HLLEDIAHVR QRLDEEARQR EEAEAAARAL

    ARFAQEAEAA RVELQKKAQA LQEECGYLRR HHQEEVGELL GQIQGCGAAQ

    AQAQAEARDA LKCDVTSALR EIRAQLEGHA VQSTLQSEEW FRVRLDRLSE

    AAKVNTDAMR SAQEEITEYR RQLQARTTEL EALKSTKESL ERQRSELEDR

    HQADIASYQD AIQQLDSELR NTKWEMAAQL REYQDLLNVK MALDIEIAAY

    RKLLEGEECR IGFGPSPFSL TEGLPKIPSI STHIKVKSEE MIKVVEKSEK

    ETVIVEGQTE EIRVTEGVTE EEDKEAQGQE GEEAEEGEEK EEEEGAAATS

    PPAEEAASPE KETKSRVKEE AKSPGEAKSP GEAKSPAEAK SPGEAKSPGE

    AKSPGEAKSP AEPKSPAEPK SPAEAKSPAE PKSPATVKSP GEAKSPSEAK

    SPAEAKSPAE AKSPAEAKSP AEAKSPAEAK SPAEAKSPAT VKSPGEAKSP

    SEAKSPAEAK SPAEAKSPAE AKSPAEVKSP GEAKSPAEPK SPAEAKSPAE

    VKSPAEAKSP AEVKSPGEAK SPAAVKSPAE AKSPAAVKSP GEAKSPGEAK

    SPAEAKSPAE AKSPIEVKSP EKAKTPVKEG AKSPAEAKSP EKAKSPVKED

    IKPPAEAKSP EKAKSPVKEG AKPPEKAKPL DVKSPEAQTP VQEEAKHPTD

    IRPPEQVKSP AKEKAKSPEK EEAKTSEKVA PKKEEVKSPV KEEVKAKEPP

    KKVEEEKTLP TPKTEAKESK KDEAPKEAPK PKVEEKKETP TEKPKDSTAE

    AKKEEAGEKK KAVASEEETP AKLGVKEEAK PKEKTETTKT EAEDTKAKEP

    SKPTETEKPK KEEMPAAPEK KDTKEEKTTE SRKPEEKPKM EAKVKEDDKS

    LSKEPSKPKT EKAEKSSSTD QKESQPPEKT TEDKATKGEK

  • UCSC uc007hvm.1; mouse.;


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