Mouse Nox1 ELISA Kit

Alternative name
Mouse Nox-1 ELISA Kit;Mouse GP91-2 ELISA Kit;Mouse MOX1 ELISA Kit;Mouse NOH-1 ELISA Kit;Mouse NOH1 ELISA Kit;Mouse NOX1a ELISA Kit;Mouse NOX1alpha ELISA Kit;Mouse NADPH oxidase 1 ELISA Kit;Mouse GP91 phox homolog ELISA Kit;Mouse NADH/NADPH mitogenic oxidase subunit p65-mox ELISA Kit;Mouse NADPH oxidase 1 alpha ELISA Kit;
Catalog
E061133
species
Mouse
GeneNox1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 9.4pg/ml
Intended UseMouse Nox1 ELISA Kit allows for the in vitro quantitative determination of Nox1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Nox1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Nox1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Nox1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Nox1 can be calculated.
Mouse NADPH oxidase 1 Protein information
Uniprot ID NOX1_MOUSE
Uniprot AC Q8CIZ9; A2AEK5; Q0KKX3; Q0KKX5; Q811U2;
UniGene Mm.233865; Mm.455133; Mm.67938;
GeneID 237038
KEGG mmu:237038;
Mouse NADPH oxidase 1 Protein SEQUENCE
SEQUENCE 591 AA; 68193 MW; B2D4AE54186A066B CRC64;
MAGELRGSRG PLQRIQIAPR EAPNLHLTMG NWLVNHWLSV LFLVSWLGLN
IFLFVYAFLN YEKSDKYYYT REILGTALAL ARASALCLNF NSMMILIPVC
RNLLSFLRGT CSFCNRTLRK PLDHNLTFHK LVAYMICIFT VIHIIAHLFN
FERYRRSQQA MDGSLASVLS SLSHPEKEDS WLNPIQSPNM TVMYAAFTSI
AGLTGVIATV ALVLMVTSAM EFIRRNYFEL FWYTHHLFIV YIICLGIHGL
GGIVRGQTEE SLGESHPHNC SHSFHEWDDH KGSCRHPHFA GHPPESWKWI
LAPIAFYIFE RILRFYRSQQ KVVITKVVMH PSNVLELQMR KRGFSMEVGQ
YIFVNCPSIS FLEWHPFTLT SAPEEEFFSV HIRAAGDWTR NLIRTFEQQH
SPMPRIEVDG PFGTVSEDVF QYEVAVLVGA GIGVTPFASI LKSIWYKFQR
ADNKLKTQKI YFYWICRETG AFAWFNNLLN SLEQEMEELG KMDFLNYRLF
LTGWDSNIAG HAALNFDRAT DILTGLKQKT SFGRPMWDNE FSRIATAHPK
SAVGVFLCGP RTLAKSLRKR CQRYSSLDPR KVQFYFNKET F
UCSC uc009ufl.2; mouse. [Q8CIZ9-1];

Category

Mouse Nox1 ELISA Kit