Human CAPS ELISA Kit

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  • Alternative name

    Human Calcyphosine ELISA Kit;

  • Catalog
    E006113
  • species
    Human
  • GeneCAPS
  • Standard CurveHuman CAPS ELISA Kit
  • Other Species Mouse CAPS ELISA KitBovine CAPS ELISA KitCanine CAPS ELISA KitRabbit CAPS ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CAPS. No significant cross-reactivity or interference between CAPS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CAPS and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CAPS ELISA Kit allows for the in vitro quantitative determination of CAPS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Intended Uses: This CAPS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CAPS. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CAPS ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CAPS antibody and an CAPS-HRP conjugate. The assay sample and buffer are incubated together with CAPS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAPS concentration since CAPS from samples and CAPS-HRP conjugate compete for the anti- CAPS antibody binding site. Since the number of sites is limited, as more sites are occupied by CAPS from the sample, fewer sites are left to bind CAPS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAPS concentration in each sample is interpolated from this standard curve.
  • Human Calcyphosin Protein information
  • Uniprot ID CAYP1_HUMAN
  • Uniprot AC Q13938; Q3MJA7; Q8WUZ3;
  • UniGene Hs.584744; Hs.627868;
  • Human Calcyphosin Protein SEQUENCE
  • SEQUENCE 189 AA; 20967 MW; E57291A996D7D1D5 CRC64;

    MDAVDATMEK LRAQCLSRGA SGIQGLARFF RQLDRDGSRS LDADEFRQGL

    AKLGLVLDQA EAEGVCRKWD RNGSGTLDLE EFLRALRPPM SQAREAVIAA

    AFAKLDRSGD GVVTVDDLRG VYSGRAHPKV RSGEWTEDEV LRRFLDNFDS

    SEKDGQVTLA EFQDYYSGVS ASMNTDEEFV AMMTSAWQL

  • UCSC uc002mdt.4; human. [Q13938-1];


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