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Mouse Ggta1 ELISA Kit

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  • Alternative name

    Mouse UDP-galactose:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase ELISA Kit;Mouse Galactosyltransferase ELISA Kit;Mouse Ggta-1 ELISA Kit;Mouse AW108479 ELISA Kit;Mouse GALT ELISA Kit;Mouse Gal ELISA Kit;Mouse Ggta ELISA Kit;Mouse alpha Gal ELISA Kit;Mouse alpha3GalT ELISA Kit;Mouse glycoprotein galactosyltransferase alpha 1, 3 ELISA Kit;Mouse N-acetyllactosaminide alpha-1,3-galactosyltransferase ELISA Kit;Mouse glycoprotein alpha galactosyl transferase 1 ELISA Kit;
  • Catalog
    E060968
  • species
    Mouse
  • GeneGgta1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GGTA1. No significant cross-reactivity or interference between GGTA1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GGTA1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse Ggta1 ELISA Kit allows for the in vitro quantitative determination of Ggta1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: GGTA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GGTA1 antibody and an GGTA1-HRP conjugate. The assay sample and buffer are incubated together with GGTA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GGTA1 concentration since GGTA1 from samples and GGTA1-HRP conjugate compete for the anti-GGTA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GGTA1 from the sample, fewer sites are left to bind GGTA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GGTA1 concentration in each sample is interpolated from this standard curve.
  • Mouse N-acetyllactosaminide alpha-1,3-galactosyltransferase Protein information
  • Uniprot ID GGTA1_MOUSE
  • Uniprot AC P23336; Q91V22;
  • UniGene Mm.281124;
  • GeneID 14594
  • KEGG mmu:14594;
  • Mouse N-acetyllactosaminide alpha-1,3-galactosyltransferase Protein SEQUENCE

  • SEQUENCE 394 AA; 46475 MW; 7766831640D1BBF7 CRC64;

    MITMLQDLHV NKISMSRSKS ETSLPSSRSG SQEKIMNVKG KVILLMLIVS

    TVVVVFWEYV NRIPEVGENR WQKDWWFPSW FKNGTHSYQE DNVEGRREKG

    RNGDRIEEPQ LWDWFNPKNR PDVLTVTPWK APIVWEGTYD TALLEKYYAT

    QKLTVGLTVF AVGKYIEHYL EDFLESADMY FMVGHRVIFY VMIDDTSRMP

    VVHLNPLHSL QVFEIRSEKR WQDISMMRMK TIGEHILAHI QHEVDFLFCM

    DVDQVFQDNF GVETLGQLVA QLQAWWYKAS PEKFTYERRE LSAAYIPFGE

    GDFYYHAAIF GGTPTHILNL TRECFKGILQ DKKHDIEAQW HDESHLNKYF

    LFNKPTKILS PEYCWDYQIG LPSDIKSVKV AWQTKEYNLV RNNV

  • UCSC uc008jkm.2; mouse. [P23336-1];



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