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  • Alternative name

    Beta-hexosaminidase subunit beta ELISA KIT; Beta-N-acetylhexosaminidase subunit beta ELISA KIT; Hexosaminidase subunit B ELISA KIT; N-acetyl-beta-glucosaminidase subunit betaHexb ELISA KIT; Hexosaminidase subunit B ELISA KIT

  • Catalog
  • species
  • GeneNAG
  • Other Species Human NAG ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse NAG. No significant cross-reactivity or interference between mouse NAG and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 3.9 uIU/ml
  • Intended UseMouse NAG ELISA Kit allows for the in vitro quantitative determination of NAG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NAG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAG bound in the initial step. The color development is stopped and the intensity of the color is measured.

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