Mouse Myod1 ELISA Kit

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  • Alternative name

    Mouse MyoD ELISA Kit;Mouse AI503393 ELISA Kit;Mouse MYF3 ELISA Kit;Mouse Myod-1 ELISA Kit;Mouse bHLHc1 ELISA Kit;Mouse myogenic differentiation 1 ELISA Kit;Mouse myoblast determination protein 1 ELISA Kit;

  • Catalog
    E060749
  • species
    Mouse
  • GeneMyod1
  • Standard CurveMouse Myod1 ELISA Kit
  • Other Species Human MYOD1 ELISA KitBovine MYOD1 ELISA KitChicken MYOD1 ELISA KitPorcine MYOD1 ELISA KitRat Myod1 ELISA KitSheep MYOD1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MYOD1. No significant cross-reactivity or interference between MYOD1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MYOD1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Myod1 ELISA Kit allows for the in vitro quantitative determination of Myod1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the assay: MYOD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MYOD1 antibody and an MYOD1-HRP conjugate. The assay sample and buffer are incubated together with MYOD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYOD1 concentration since MYOD1 from samples and MYOD1-HRP conjugate compete for the anti-MYOD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MYOD1 from the sample, fewer sites are left to bind MYOD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYOD1 concentration in each sample is interpolated from this standard curve.
  • Mouse Myoblast determination protein 1 Protein information
  • Uniprot ID MYOD1_MOUSE
  • Uniprot AC P10085; Q8C6B1;
  • UniGene Mm.1526;
  • GeneID 17927
  • KEGG mmu:17927;
  • Mouse Myoblast determination protein 1 Protein SEQUENCE
  • SEQUENCE 318 AA; 34233 MW; AEC6572277A78D54 CRC64;

    MELLSPPLRD IDLTGPDGSL CSFETADDFY DDPCFDSPDL RFFEDLDPRL

    VHMGALLKPE EHAHFPTAVH PGPGAREDEH VRAPSGHHQA GRCLLWACKA

    CKRKTTNADR RKAATMRERR RLSKVNEAFE TLKRCTSSNP NQRLPKVEIL

    RNAIRYIEGL QALLRDQDAA PPGAAAFYAP GPLPPGRGSE HYSGDSDASS

    PRSNCSDGMM DYSGPPSGPR RQNGYDTAYY SEAARESRPG KSAAVSSLDC

    LSSIVERIST DSPAAPALLL ADAPPESPPG PPEGASLSDT EQGTQTPSPD

    AAPQCPAGSN PNAIYQVL

  • UCSC uc012fkv.1; mouse.;


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