Human CAMK2A ELISA Kit

Alternative name
Human caM kinase II subunit alpha ELISA Kit;Human caMK-II subunit alpha ELISA Kit;Human CAMKA ELISA Kit;Human KIAA0968 ELISA Kit;Human calcium/calmodulin dependent protein kinase II alpha ELISA Kit;Human calcium/calmodulin-dependent protein kinase type II subunit alpha ELISA Kit;Human CaM kinase II alpha subunit ELISA Kit;Human CaM-kinase II alpha chain ELISA Kit;Human CaMK-II alpha subunit ELISA Kit;Human CaMKIINalpha ELISA Kit;Human calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha ELISA Kit;Human calcium/calmodulin-dependent protein kinase II alpha-B subunit ELISA Kit;Human calcium/calmodulin-dependent protein kinase type II alpha chain ELISA Kit;
Catalog
E006071
species
Human
GeneCAMK2A
Standard Curve
Other Species Mouse Camk2a ELISA Kit Rat Camk2a ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a). No significant cross-reactivity or interference between Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.064ng/mL.
Intended UseHuman CAMK2A ELISA Kit allows for the in vitro quantitative determination of CAMK2A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Calcium/calmodulin-dependent protein kinase type II subunit alpha Protein information
Uniprot ID KCC2A_HUMAN
Uniprot AC Q9UQM7; Q9UL21; Q9Y2H4; Q9Y352;
UniGene Hs.743976;
GeneID 815
KEGG hsa:815;
Human Calcium/calmodulin-dependent protein kinase type II subunit alpha Protein SEQUENCE
SEQUENCE 478 AA; 54088 MW; 208143A311BA9262 CRC64;
MATITCTRFT EEYQLFEELG KGAFSVVRRC VKVLAGQEYA AKIINTKKLS
ARDHQKLERE ARICRLLKHP NIVRLHDSIS EEGHHYLIFD LVTGGELFED
IVAREYYSEA DASHCIQQIL EAVLHCHQMG VVHRDLKPEN LLLASKLKGA
AVKLADFGLA IEVEGEQQAW FGFAGTPGYL SPEVLRKDPY GKPVDLWACG
VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK
MLTINPSKRI TAAEALKHPW ISHRSTVASC MHRQETVDCL KKFNARRKLK
GAILTTMLAT RNFSGGKSGG NKKSDGVKES SESTNTTIED EDTKVRKQEI
IKVTEQLIEA ISNGDFESYT KMCDPGMTAF EPEALGNLVE GLDFHRFYFE
NLWSRNSKPV HTTILNPHIH LMGDESACIA YIRITQYLDA GGIPRTAQSE
ETRVWHRRDG KWQIVHFHRS GAPSVLPH
UCSC uc003lrt.3; human. [Q9UQM7-1];

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Human CAMK2A ELISA Kit