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  • Alternative name

    Calcium/calmodulin-dependent protein kinase type II subunit beta ELISA KIT; CAMK2B ELISA KIT; CAM2 ELISA KIT; CAMK2 ELISA KIT; CAMKB ELISA KIT; CaM kinase II subunit beta ELISA KIT; CaMK-II subunit beta ELISA KIT

  • Catalog
  • species
  • GeneCAMK2
  • Standard CurveHuman CAMK2 ELISA Kit
  • Other Species Mouse CAMK2 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CAMK2 ELISA Kit allows for the in vitro quantitative determination of CAMK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This CAMK2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CAMK2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY CAMK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CAMK2 antibody and an CAMK2-HRP conjugate. The assay sample and buffer are incubated together with CAMK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAMK2 concentration since CAMK2 from samples and CAMK2-HRP conjugate compete for the anti-CAMK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAMK2 from the sample, fewer sites are left to bind CAMK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAMK2 concentration in each sample is interpolated from this standard curve.

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