Human CASR ELISA Kit

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  • Alternative name

    Human CaSR ELISA Kit;Human parathyroid cell calcium-sensing receptor 1 ELISA Kit;Human PCAR1 ELISA Kit;Human GPRC2A ELISA Kit;Human CAR ELISA Kit;Human EIG8 ELISA Kit;Human FHH ELISA Kit;Human FIH ELISA Kit;Human HHC ELISA Kit;Human HHC1 ELISA Kit;Human HYPOC1 ELISA Kit;Human NSHPT ELISA Kit;Human calcium sensing receptor ELISA Kit;Human extracellular calcium-sensing receptor ELISA Kit;Human parathyroid Ca(2+)-sensing receptor 1 ELISA Kit;

  • Catalog
    E006059
  • species
    Human
  • GeneCASR
  • Other Species Mouse Casr ELISA KitPorcine CASR ELISA KitRat Casr ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CASR ELISA Kit allows for the in vitro quantitative determination of CASR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: CaSR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CaSR. Standards or samples are then added to the microtiter plate wells and CaSR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CaSR present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CaSR are added to each well to "sandwich" the CaSR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CaSR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CaSR concentration in each sample is interpolated from this standard curve.
  • Human Extracellular calcium-sensing receptor Protein information
  • Uniprot ID CASR_HUMAN
  • Uniprot AC P41180; Q13912; Q16108; Q16109; Q16110; Q16379; Q2M1T0; Q4PJ19;
  • UniGene Hs.435615;
  • GeneID 846
  • KEGG hsa:846;
  • Human Extracellular calcium-sensing receptor Protein SEQUENCE
  • SEQUENCE 1078 AA; 120674 MW; 620C57DB1A83E1DB CRC64;

    MAFYSCCWVL LALTWHTSAY GPDQRAQKKG DIILGGLFPI HFGVAAKDQD

    LKSRPESVEC IRYNFRGFRW LQAMIFAIEE INSSPALLPN LTLGYRIFDT

    CNTVSKALEA TLSFVAQNKI DSLNLDEFCN CSEHIPSTIA VVGATGSGVS

    TAVANLLGLF YIPQVSYASS SRLLSNKNQF KSFLRTIPND EHQATAMADI

    IEYFRWNWVG TIAADDDYGR PGIEKFREEA EERDICIDFS ELISQYSDEE

    EIQHVVEVIQ NSTAKVIVVF SSGPDLEPLI KEIVRRNITG KIWLASEAWA

    SSSLIAMPQY FHVVGGTIGF ALKAGQIPGF REFLKKVHPR KSVHNGFAKE

    FWEETFNCHL QEGAKGPLPV DTFLRGHEES GDRFSNSSTA FRPLCTGDEN

    ISSVETPYID YTHLRISYNV YLAVYSIAHA LQDIYTCLPG RGLFTNGSCA

    DIKKVEAWQV LKHLRHLNFT NNMGEQVTFD ECGDLVGNYS IINWHLSPED

    GSIVFKEVGY YNVYAKKGER LFINEEKILW SGFSREVPFS NCSRDCLAGT

    RKGIIEGEPT CCFECVECPD GEYSDETDAS ACNKCPDDFW SNENHTSCIA

    KEIEFLSWTE PFGIALTLFA VLGIFLTAFV LGVFIKFRNT PIVKATNREL

    SYLLLFSLLC CFSSSLFFIG EPQDWTCRLR QPAFGISFVL CISCILVKTN

    RVLLVFEAKI PTSFHRKWWG LNLQFLLVFL CTFMQIVICV IWLYTAPPSS

    YRNQELEDEI IFITCHEGSL MALGFLIGYT CLLAAICFFF AFKSRKLPEN

    FNEAKFITFS MLIFFIVWIS FIPAYASTYG KFVSAVEVIA ILAASFGLLA

    CIFFNKIYII LFKPSRNTIE EVRCSTAAHA FKVAARATLR RSNVSRKRSS

    SLGGSTGSTP SSSISSKSNS EDPFPQPERQ KQQQPLALTQ QEQQQQPLTL

    PQQQRSQQQP RCKQKVIFGS GTVTFSLSFD EPQKNAMAHR NSTHQNSLEA

    QKSSDTLTRH QPLLPLQCGE TDLDLTVQET GLQGPVGGDQ RPEVEDPEEL

    SPALVVSSSQ SFVISGGGST VTENVVNS

  • UCSC uc003eev.5; human. [P41180-1];


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