Human CASR ELISA Kit

Alternative name
Human CaSR ELISA Kit;Human parathyroid cell calcium-sensing receptor 1 ELISA Kit;Human PCAR1 ELISA Kit;Human GPRC2A ELISA Kit;Human CAR ELISA Kit;Human EIG8 ELISA Kit;Human FHH ELISA Kit;Human FIH ELISA Kit;Human HHC ELISA Kit;Human HHC1 ELISA Kit;Human HYPOC1 ELISA Kit;Human NSHPT ELISA Kit;Human calcium sensing receptor ELISA Kit;Human extracellular calcium-sensing receptor ELISA Kit;Human parathyroid Ca(2+)-sensing receptor 1 ELISA Kit;
Catalog
E006059
species
Human
GeneCASR
Other Species Mouse Casr ELISA Kit Porcine CASR ELISA Kit Rat Casr ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CASR ELISA Kit allows for the in vitro quantitative determination of CASR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: CaSR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CaSR. Standards or samples are then added to the microtiter plate wells and CaSR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CaSR present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CaSR are added to each well to "sandwich" the CaSR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CaSR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CaSR concentration in each sample is interpolated from this standard curve.
Human Extracellular calcium-sensing receptor Protein information
Uniprot ID CASR_HUMAN
Uniprot AC P41180; Q13912; Q16108; Q16109; Q16110; Q16379; Q2M1T0; Q4PJ19;
UniGene Hs.435615;
GeneID 846
KEGG hsa:846;
Human Extracellular calcium-sensing receptor Protein SEQUENCE
SEQUENCE 1078 AA; 120674 MW; 620C57DB1A83E1DB CRC64;
MAFYSCCWVL LALTWHTSAY GPDQRAQKKG DIILGGLFPI HFGVAAKDQD
LKSRPESVEC IRYNFRGFRW LQAMIFAIEE INSSPALLPN LTLGYRIFDT
CNTVSKALEA TLSFVAQNKI DSLNLDEFCN CSEHIPSTIA VVGATGSGVS
TAVANLLGLF YIPQVSYASS SRLLSNKNQF KSFLRTIPND EHQATAMADI
IEYFRWNWVG TIAADDDYGR PGIEKFREEA EERDICIDFS ELISQYSDEE
EIQHVVEVIQ NSTAKVIVVF SSGPDLEPLI KEIVRRNITG KIWLASEAWA
SSSLIAMPQY FHVVGGTIGF ALKAGQIPGF REFLKKVHPR KSVHNGFAKE
FWEETFNCHL QEGAKGPLPV DTFLRGHEES GDRFSNSSTA FRPLCTGDEN
ISSVETPYID YTHLRISYNV YLAVYSIAHA LQDIYTCLPG RGLFTNGSCA
DIKKVEAWQV LKHLRHLNFT NNMGEQVTFD ECGDLVGNYS IINWHLSPED
GSIVFKEVGY YNVYAKKGER LFINEEKILW SGFSREVPFS NCSRDCLAGT
RKGIIEGEPT CCFECVECPD GEYSDETDAS ACNKCPDDFW SNENHTSCIA
KEIEFLSWTE PFGIALTLFA VLGIFLTAFV LGVFIKFRNT PIVKATNREL
SYLLLFSLLC CFSSSLFFIG EPQDWTCRLR QPAFGISFVL CISCILVKTN
RVLLVFEAKI PTSFHRKWWG LNLQFLLVFL CTFMQIVICV IWLYTAPPSS
YRNQELEDEI IFITCHEGSL MALGFLIGYT CLLAAICFFF AFKSRKLPEN
FNEAKFITFS MLIFFIVWIS FIPAYASTYG KFVSAVEVIA ILAASFGLLA
CIFFNKIYII LFKPSRNTIE EVRCSTAAHA FKVAARATLR RSNVSRKRSS
SLGGSTGSTP SSSISSKSNS EDPFPQPERQ KQQQPLALTQ QEQQQQPLTL
PQQQRSQQQP RCKQKVIFGS GTVTFSLSFD EPQKNAMAHR NSTHQNSLEA
QKSSDTLTRH QPLLPLQCGE TDLDLTVQET GLQGPVGGDQ RPEVEDPEEL
SPALVVSSSQ SFVISGGGST VTENVVNS
UCSC uc003eev.5; human. [P41180-1];

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Human CASR ELISA Kit