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Alternative name
Mucin-7 ELISA KIT; Apo-MG2 ELISA KIT; Salivary mucin-7MUC7 ELISA KIT; MG2 ELISA KIT; MUC-7 ELISA KIT
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Catalog
E060525
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species
Mouse
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GeneMUC7
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse MUC7. No significant cross-reactivity or interference between Mouse MUC7 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse MUC7 and all the analogues, therefore, cross reaction may still exist.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse MUC7 ELISA Kit allows for the in vitro quantitative determination of MUC7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MUC7 in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MUC7. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse MUC7 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MUC7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse MUC7. You can calculate the concentration of Mouse MUC7 in the samples by comparing the OD of the samples to the standard curve.
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