Mouse MCP-5 ELISA Kit

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  • Alternative name

    Mcp-5 ELISA KIT; Mcp-5mcp-5 ELISA KIT; KK9_0714 ELISA KIT

  • Catalog
    E060437
  • species
    Mouse
  • GeneMCP-5
  • Other Species Human MCP 5 ELISA KitMouse CCL12/MCP-5 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity16.82 pg/ml
  • Intended UseMouse MCP-5 ELISA Kit allows for the in vitro quantitative determination of MCP-5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    For samples: For the quantitative determination of mouse monocyte chemotactic protein 5 (MCP-5) concentrations in serum, plasma, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCP-5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP-5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCP-5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCP-5 bound in the initial step. The color development is stopped and the intensity of the color is measured.



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