Mouse Msn ELISA Kit

Alternative name
Mouse membrane-organizing extension spike protein ELISA Kit;Mouse C78546 ELISA Kit;Mouse moesin ELISA Kit;
Catalog
E060382
species
Mouse
GeneMsn
SpecificityThis assay has high sensitivity and excellent specificity for detection of MSN. No significant cross-reactivity or interference between MSN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MSN and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Msn ELISA Kit allows for the in vitro quantitative determination of Msn , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: MSN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MSN antibody and an MSN-HRP conjugate. The assay sample and buffer are incubated together with MSN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MSN concentration since MSN from samples and MSN-HRP conjugate compete for the anti-MSN antibody binding site. Since the number of sites is limited, as more sites are occupied by MSN from the sample, fewer sites are left to bind MSN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MSN concentration in each sample is interpolated from this standard curve.
Mouse Moesin Protein information
Uniprot ID MOES_MOUSE
Uniprot AC P26041; B1AX70; Q3UL28; Q8BSN4;
UniGene Mm.138876;
GeneID 17698
KEGG mmu:17698;
Mouse Moesin Protein SEQUENCE
SEQUENCE 577 AA; 67767 MW; 4E9B5521313EF5B5 CRC64;
MPKTISVRVT TMDAELEFAI QPNTTGKQLF DQVVKTIGLR EVWFFGLQYQ
DTKAFSTWLK LNKKVTAQDV RKESPLLFKF RAKFYPEDVS EELIQDITQR
LFFLQVKEGI LNDDIYCPPE TAVLLASYAV QSKYGDFNKE VHKSGYLAGD
KLLPQRVLEQ HKLNKDQWEE RIQVWHEEHR GMLREDAVLE YLKIAQDLEM
YGVNYFSIKN KKGSELWLGV DALGLNIYEQ NDRLTPKIGF PWSEIRNISF
NDKKFVIKPI DKKAPDFVFY APRLRINKRI LALCMGNHEL YMRRRKPDTI
EVQQMKAQAR EEKHQKQMER ALLENEKKKR ELAEKEKEKI EREKEELMEK
LKQIEEQTKK AQQELEEQTR RALELEQERK RAQSEAEKLA KERQEAEEAK
EALLQASRDQ KKTQEQLASE MAELTARISQ LEMARKKKES EAVEWQQKAQ
MVQEDLEKTR AELKTAMSTP HVAEPAENEH DEQDENGAEA SAELRADAMA
KDRSEEERTT EAEKNERVQK HLKALTSELA NARDESKKTA NDMIHAENMR
LGRDKYKTLR QIRQGNTKQR IDEFESM
UCSC uc009tug.1; mouse.;

Category

Mouse Msn ELISA Kit

Mouse membrane-organizing extension spike protein ELISA Kit;Mouse C78546 ELISA Kit;Mouse moesin ELISA Kit;