Mouse Ucp3 ELISA Kit

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  • Alternative name

    Mouse UCP 3 ELISA Kit;Mouse solute carrier family 25 member 9 ELISA Kit;Mouse Slc25a9 ELISA Kit;Mouse AI645527 ELISA Kit;Mouse UCP-3 ELISA Kit;Mouse uncoupling protein 3 (mitochondrial, proton carrier) ELISA Kit;Mouse mitochondrial uncoupling protein 3 ELISA Kit;Mouse uncoupling protein 3, mitochondrial ELISA Kit;

  • Catalog
    E060264
  • species
    Mouse
  • GeneUcp3
  • Standard CurveMouse Ucp3 ELISA Kit
  • Other Species Human UCP3 ELISA KitBovine UCP3 ELISA KitCanine UCP3 ELISA KitPorcine UCP3 ELISA KitRat Ucp3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of UCP3. No significant cross-reactivity or interference between UCP3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between UCP3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseMouse Ucp3 ELISA Kit allows for the in vitro quantitative determination of Ucp3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: UCP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-UCP3 antibody and an UCP3-HRP conjugate. The assay sample and buffer are incubated together with UCP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UCP3 concentration since UCP3 from samples and UCP3-HRP conjugate compete for the anti-UCP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by UCP3 from the sample, fewer sites are left to bind UCP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UCP3 concentration in each sample is interpolated from this standard curve.
  • Mouse Mitochondrial uncoupling protein 3 Protein information
  • Uniprot ID UCP3_MOUSE
  • Uniprot AC P56501; O88293;
  • UniGene Mm.6254;
  • GeneID 22229
  • KEGG mmu:22229;
  • Mouse Mitochondrial uncoupling protein 3 Protein SEQUENCE
  • SEQUENCE 308 AA; 33911 MW; 12CAD7674DF7D0C3 CRC64;

    MVGLQPSEVP PTTVVKFLGA GTAACFADLL TFPLDTAKVR LQIQGENPGA

    QSVQYRGVLG TILTMVRTEG PRSPYSGLVA GLHRQMSFAS IRIGLYDSVK

    QFYTPKGADH SSVAIRILAG CTTGAMAVTC AQPTDVVKVR FQAMIRLGTG

    GERKYRGTMD AYRTIAREEG VRGLWKGTWP NITRNAIVNC AEMVTYDIIK

    EKLLESHLFT DNFPCHFVSA FGAGFCATVV ASPVDVVKTR YMNAPLGRYR

    SPLHCMLKMV AQEGPTAFYK GFVPSFLRLG AWNVMMFVTY EQLKRALMKV

    QVLRESPF

  • UCSC uc009ina.1; mouse.;


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