Mouse MK ELISA Kit

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  • Alternative name

    Midkine ELISA KIT; Amphiregulin-associated protein ELISA KIT; ARAP ELISA KIT; Midgestation and kidney protein ELISA KIT; Neurite outgrowth-promoting factor 2 ELISA KIT; Neurite outgrowth-promoting proteinMDK ELISA KIT; MK1 ELISA KIT; NEGF2 ELISA KIT; MK ELISA KIT; ARAP ELISA KIT

  • Catalog
  • species
  • GeneMK
  • Standard CurveMouse MK ELISA Kit
  • Other Species Human MK ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Midkine (MK). No significant cross-reactivity or interference between Midkine (MK) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.054ng/mL.
  • Intended UseMouse MK ELISA Kit allows for the in vitro quantitative determination of MK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Midkine (MK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Midkine (MK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Midkine (MK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Midkine (MK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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