Mouse MG ELISA Kit

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  • Alternative name

    Methylglyoxal synthase ELISA KIT; mgsA ELISA KIT; Marky_0332 ELISA KIT; MGS ELISA KIT

  • Catalog
    E060033
  • species
    Mouse
  • GeneMG
  • Standard CurveMouse MG ELISA Kit
  • Other Species Human A1 MG ELISA KitHuman A1-MG ELISA KitHuman A2-MG ELISA KitHuman a2 MG ELISA KitHuman Alpha 1 MG ELISA KitHuman Pre Alpha 2 MG ELISA KitHuman alpha1-MG ELISA KitHuman alpha2 MG ELISA KitHuman PRE-alpha2 MG ELISA KitHuman alpha2-MG ELISA KitHuman beta2-MG ELISA KitHuman beta2 MG ELISA KitHuman BMG/beta2-MG ELISA KitHuman MG ELISA KitHuman Mg (2+) ATPase ELISA KitMouse A1-MG ELISA KitMouse a2-MG ELISA KitMouse alpha1-MG ELISA KitMouse Pre-alpha2 MG ELISA KitMouse alpha2-MG ELISA KitMouse beta2-MG ELISA KitMouse BMG/beta2-MG ELISA KitMouse Mg (2+) -ATPase ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MG. No significant cross-reactivity or interference between MG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MG and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse MG ELISA Kit allows for the in vitro quantitative determination of MG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses||This MG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MG. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: MG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MG antibody and an MG-HRP conjugate. The assay sample and buffer are incubated together with MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MG concentration since MG from samples and MG-HRP conjugate compete for the anti-MG antibody binding site. Since the number of sites is limited, as more sites are occupied by MG from the sample, fewer sites are left to bind MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MG concentration in each sample is interpolated from this standard curve.



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