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Alternative name
Metallothionein-3 ELISA KIT; GIFB ELISA KIT; GIF ELISA KIT; Growth inhibitory factor ELISA KIT; Metallothionein-III ELISA KIT; MT-IIIMT3 ELISA KIT; MT-3 ELISA KIT; GIF ELISA KIT; MT-III ELISA KIT
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Catalog
E059961
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species
Mouse
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GeneMT-3
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Standard Curve
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Other Species
Human MT 3 ELISA Kit
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse MT-3 ELISA Kit allows for the in vitro quantitative determination of MT-3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyMicrobiology
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Product Description
specificalFor Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Intended Uses: This MET3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MET3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: MET3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MET3 antibody and an MET3-HRP conjugate. The assay sample and buffer are incubated together with MET3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MET3 concentration since MET3 from samples and MET3-HRP conjugate compete for the anti-MET3 antibody binding site. Since the number of sites is limited, as more sites are occupied by MET3 from the sample, fewer sites are left to bind MET3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MET3 concentration in each sample is interpolated from this standard curve.
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