Mouse Msln ELISA Kit

Alternative name
Mouse pre-pro-megakaryocyte-potentiating factor ELISA Kit;Mouse MPF ELISA Kit;Mouse Mes ELISA Kit;Mouse mesothelin ELISA Kit;Mouse megakaryocyte potentiating factor ELISA Kit;
Catalog
E059894
species
Mouse
GeneMsln
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseMouse Msln ELISA Kit allows for the in vitro quantitative determination of Msln , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Msln antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Msln antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Msln amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Msln can be calculated.
Mouse Mesothelin, cleaved form Protein information
Uniprot ID MSLN_MOUSE
Uniprot AC Q61468;
UniGene Mm.17510;
GeneID 56047
KEGG mmu:56047;
Mouse Mesothelin, cleaved form Protein SEQUENCE
SEQUENCE 625 AA; 69423 MW; 9A5E9C3222C6983F CRC64;
MALPTARPLL GSCGSPICSR SFLLLLLSLG WIPRLQTQTT KTSQEATLLH
AVNGAADFAS LPTGLFLGLT CEEVSDLSME QAKGLAMAVR QKNITLRGHQ
LRCLARRLPR HLTDEELNAL PLDLLLFLNP AMFPGQQACA HFFSLISKAN
VDVLPRRSLE RQRLLMEALK CQGVYGFQVS EADVRALGGL ACDLPGKFVA
RSSEVLLPWL AGCQGPLDQS QEKAVREVLR SGRTQYGPPS KWSVSTLDAL
QSLVAVLDES IVQSIPKDVK AEWLQHISRD PSRLGSKLTV IHPRFRRDAE
QKACPPGKEP YKVDEDLIFY QNWELEACVD GTMLARQMDL VNEIPFTYEQ
LSIFKHKLDK TYPQGYPESL IQQLGHFFRY VSPEDIHQWN VTSPDTVKTL
LKVSKGQKMN AQAIALVACY LRGGGQLDED MVKALGDIPL SYLCDFSPQD
LHSVPSSVMW LVGPQDLDKC SQRHLGLLYQ KACSAFQNVS GLEYFEKIKT
FLGGASVKDL RALSQHNVSM DIATFKRLQV DSLVGLSVAE VQKLLGPNIV
DLKTEEDKSP VRDWLFRQHQ KDLDRLGLGL QGGIPNGYLV LDFNVREAFS
SRASLLGPGF VLIWIPALLP ALRLS
UCSC uc008bbo.1; mouse.;

Category

Mouse Msln ELISA Kit

Mouse pre-pro-megakaryocyte-potentiating factor ELISA Kit;Mouse MPF ELISA Kit;Mouse Mes ELISA Kit;Mouse mesothelin ELISA Kit;Mouse megakaryocyte potentiating factor ELISA Kit;