Mouse Mest ELISA Kit

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  • Alternative name

    Mouse paternally-expressed gene 1 protein ELISA Kit;Mouse Peg1 ELISA Kit;Mouse AA408879 ELISA Kit;Mouse AI256745 ELISA Kit;Mouse mesoderm specific transcript ELISA Kit;Mouse mesoderm-specific transcript protein ELISA Kit;

  • Catalog
    E059892
  • species
    Mouse
  • GeneMest
  • Other Species Human MEST ELISA KitBovine MEST ELISA KitRat Mest ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse MEST. No significant cross-reactivity or interference between mouse MEST and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of mouse MEST is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseMouse Mest ELISA Kit allows for the in vitro quantitative determination of Mest , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MEST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MEST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEST is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEST bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Mouse Mesoderm-specific transcript protein Protein information
  • Uniprot ID MEST_MOUSE
  • Uniprot AC Q07646; Q792T8; Q8BS88; Q8K463; Q99KT5;
  • UniGene Mm.335639;
  • GeneID 17294
  • KEGG mmu:17294;
  • Mouse Mesoderm-specific transcript protein Protein SEQUENCE
  • SEQUENCE 335 AA; 38907 MW; F5F394F46152E712 CRC64;

    MVRRDRLRRM REWWVQVGLL AVPLLAAYLH IPPPQLSPAL HSWKTSGKFF

    TYKGLRIFYQ DSVGVVGSPE IVVLLHGFPT SSYDWYKIWE GLTLRFHRVI

    ALDFLGFGFS DKPRPHQYSI FEQASIVESL LRHLGLQNRR INLLSHDYGD

    IVAQELLYRY KQNRSGRLTI KSLCLSNGGI FPETHRPLLL QKLLKDGGVL

    SPILTRLMNF FVFSRGLTPV FGPYTRPTES ELWDMWAVIR NNDGNLVIDS

    LLQYINQRKK FRRRWVGALA SVSIPIHFIY GPLDPINPYP EFLELYRKTL

    PRSTVSILDD HISHYPQLED PMGFLNAYMG FINSF

  • UCSC uc009bfs.2; mouse. [Q07646-1];


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