Mouse Mest ELISA Kit

Alternative name
Mouse paternally-expressed gene 1 protein ELISA Kit;Mouse Peg1 ELISA Kit;Mouse AA408879 ELISA Kit;Mouse AI256745 ELISA Kit;Mouse mesoderm specific transcript ELISA Kit;Mouse mesoderm-specific transcript protein ELISA Kit;
Catalog
E059892
species
Mouse
GeneMest
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse MEST. No significant cross-reactivity or interference between mouse MEST and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of mouse MEST is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseMouse Mest ELISA Kit allows for the in vitro quantitative determination of Mest , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MEST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MEST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEST is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEST bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Mesoderm-specific transcript protein Protein information
Uniprot ID MEST_MOUSE
Uniprot AC Q07646; Q792T8; Q8BS88; Q8K463; Q99KT5;
UniGene Mm.335639;
GeneID 17294
KEGG mmu:17294;
Mouse Mesoderm-specific transcript protein Protein SEQUENCE
SEQUENCE 335 AA; 38907 MW; F5F394F46152E712 CRC64;
MVRRDRLRRM REWWVQVGLL AVPLLAAYLH IPPPQLSPAL HSWKTSGKFF
TYKGLRIFYQ DSVGVVGSPE IVVLLHGFPT SSYDWYKIWE GLTLRFHRVI
ALDFLGFGFS DKPRPHQYSI FEQASIVESL LRHLGLQNRR INLLSHDYGD
IVAQELLYRY KQNRSGRLTI KSLCLSNGGI FPETHRPLLL QKLLKDGGVL
SPILTRLMNF FVFSRGLTPV FGPYTRPTES ELWDMWAVIR NNDGNLVIDS
LLQYINQRKK FRRRWVGALA SVSIPIHFIY GPLDPINPYP EFLELYRKTL
PRSTVSILDD HISHYPQLED PMGFLNAYMG FINSF
UCSC uc009bfs.2; mouse. [Q07646-1];

Category

Mouse Mest ELISA Kit

Mouse paternally-expressed gene 1 protein ELISA Kit;Mouse Peg1 ELISA Kit;Mouse AA408879 ELISA Kit;Mouse AI256745 ELISA Kit;Mouse mesoderm specific transcript ELISA Kit;Mouse mesoderm-specific transcript protein ELISA Kit;