Mouse Manf ELISA Kit

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  • Alternative name

    Mouse Arginine-rich protein ELISA Kit;Mouse Protein ARMET ELISA Kit;Mouse Armet ELISA Kit;

  • Catalog
    E059884
  • species
    Mouse
  • GeneManf
  • Standard CurveMouse Manf ELISA Kit
  • Other Species Human MANF ELISA KitBovine MANF ELISA KitRat Manf ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MANF. No significant cross-reactivity or interference between MANF and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of MANF is typically less than 5.7pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two s
  • Intended UseMouse Manf ELISA Kit allows for the in vitro quantitative determination of Manf , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MANF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MANF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MANF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MANF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Mesencephalic astrocyte-derived neurotrophic factor Protein information
  • Uniprot ID MANF_MOUSE
  • Uniprot AC Q9CXI5;
  • UniGene Mm.29778;
  • Mouse Mesencephalic astrocyte-derived neurotrophic factor Protein SEQUENCE
  • SEQUENCE 179 AA; 20374 MW; E5BCEE8C033C1530 CRC64;

    MWATRGLAVA LALSVLPDSR ALRPGDCEVC ISYLGRFYQD LKDRDVTFSP

    ATIEEELIKF CREARGKENR LCYYIGATDD AATKIINEVS KPLAHHIPVE

    KICEKLKKKD SQICELKYDN QIDLSTVDLK KLRVKELKKI LDDWGEMCKG

    CAEKSDYIRK INELMPKYAP KAASARTDL



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