SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse MIA. No significant cross-reactivity or interference between Mouse MIA and analogues was observed.
Intended UseMouse Mia ELISA Kit allows for the in vitro quantitative determination of Mia , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Melanoma-derived growth regulatory protein Protein information
Uniprot ID
MIA_MOUSE
Uniprot AC
Q61865; O09086; P97495;
UniGene
Mm.262447;
GeneID
12587
KEGG
mmu:12587;
Mouse Melanoma-derived growth regulatory protein Protein SEQUENCE