Mouse Mmp2 ELISA Kit

Alternative name
Mouse 72 kDa gelatinase ELISA Kit;Mouse gelatinase A ELISA Kit;Mouse Matrix metalloproteinase-2 ELISA Kit;Mouse MMP-2 ELISA Kit;Mouse Clg4a ELISA Kit;Mouse GelA ELISA Kit;Mouse matrix metallopeptidase 2 ELISA Kit;Mouse 72 kDa type IV collagenase ELISA Kit;Mouse 72kD gelatinase ELISA Kit;Mouse 72kD type IV collagenase ELISA Kit;Mouse 72kDa gelatinase ELISA Kit;Mouse 72kDa type IV collagenase ELISA Kit;Mouse matrix metalloproteinase 2 ELISA Kit;
Catalog
E059573
species
Mouse
GeneMmp2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse MMP2. No significant cross-reactivity or interference between Mouse MMP2 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity3.9 pg/ml
Intended UseMouse Mmp2 ELISA Kit allows for the in vitro quantitative determination of Mmp2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: 72 kDa type IV collagenase also known as matrixmetalloproteinase-2 (MMP-2) and gelatinase A is an enzyme thatin humans is encoded by the MMP2 gene.Proteins of the matrix metalloproteinase (MMP) family are involvedin the breakdown of extracellular matrix in normal physiologicalprocesses, such as embryonic development, reproduction, andtissue remodeling, as well as in disease processes, such asarthritis and metastasis. Most MMP's are secreted as inactiveproproteins which are activated when cleaved by extracellularproteinases. This gene encodes an enzyme which degrades typeIV collagen, the major structural component of basementmembranes. The enzyme plays a role in endometrial menstrualbreakdown, regulation of vascularization and the inflammatoryresponse Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-2. Standards or samples are then addedto the appropriate microtiter plate wells with a biotin-conjugatedantibody preparation specific for MMP-2 and Avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate welland incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine)substrate solution is added to each well. Only those wells thatcontain MMP-2, biotin-conjugated antibody andenzyme-conjugated Avidin will exhibit a change in color. Theenzyme-substrate reaction is terminated by the addition of asulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of MMP-2 in the samples is then determined bycomparing the O.D. of the samples to the standard curve.
Mouse PEX Protein information
Uniprot ID MMP2_MOUSE
Uniprot AC P33434;
UniGene Mm.29564;
GeneID 17390
KEGG mmu:17390;
Mouse PEX Protein SEQUENCE
SEQUENCE 662 AA; 74102 MW; C630A7DBDB272F02 CRC64;
MEARVAWGAL AGPLRVLCVL CCLLGRAIAA PSPIIKFPGD VAPKTDKELA
VQYLNTFYGC PKESCNLFVL KDTLKKMQKF FGLPQTGDLD QNTIETMRKP
RCGNPDVANY NFFPRKPKWD KNQITYRIIG YTPDLDPETV DDAFARALKV
WSDVTPLRFS RIHDGEADIM INFGRWEHGD GYPFDGKDGL LAHAFAPGTG
VGGDSHFDDD ELWTLGEGQV VRVKYGNADG EYCKFPFLFN GREYSSCTDT
GRSDGFLWCS TTYNFEKDGK YGFCPHEALF TMGGNADGQP CKFPFRFQGT
SYNSCTTEGR TDGYRWCGTT EDYDRDKKYG FCPETAMSTV GGNSEGAPCV
FPFTFLGNKY ESCTSAGRND GKVWCATTTN YDDDRKWGFC PDQGYSLFLV
AAHEFGHAMG LEHSQDPGAL MAPIYTYTKN FRLSHDDIKG IQELYGPSPD
ADTDTGTGPT PTLGPVTPEI CKQDIVFDGI AQIRGEIFFF KDRFIWRTVT
PRDKPTGPLL VATFWPELPE KIDAVYEAPQ EEKAVFFAGN EYWVYSASTL
ERGYPKPLTS LGLPPDVQQV DAAFNWSKNK KTYIFAGDKF WRYNEVKKKM
DPGFPKLIAD SWNAIPDNLD AVVDLQGGGH SYFFKGAYYL KLENQSLKSV
KFGSIKSDWL GC
UCSC uc009mue.1; mouse. [P33434-1];

Category

Mouse Mmp2 ELISA Kit