Mouse Gp49a ELISA Kit

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  • Alternative name

    Mouse gp49 ELISA Kit;Mouse Gp49a ELISA Kit;Mouse leukocyte immunoglobulin-like receptor, subfamily B, member 4B ELISA Kit;Mouse mast cell surface glycoprotein Gp49A ELISA Kit;Mouse glycoprotein 49 A ELISA Kit;

  • Catalog
    E059529
  • species
    Mouse
  • GeneGp49a
  • Standard CurveMouse Gp49a ELISA Kit
  • Other Species Human Gp49a ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural mouse Mast cell surface glycoprotein Gp49A. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Gp49a ELISA Kit allows for the in vitro quantitative determination of Gp49a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mast cell surface glycoprotein Gp49A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mast cell surface glycoprotein Gp49A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mast cell surface glycoprotein Gp49A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mast cell surface glycoprotein Gp49A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Mast cell surface glycoprotein Gp49A Protein information
  • Uniprot ID GP49A_MOUSE
  • Uniprot AC Q61450;
  • UniGene Mm.439661;
  • GeneID 14727
  • KEGG mmu:14727;
  • Mouse Mast cell surface glycoprotein Gp49A Protein SEQUENCE
  • SEQUENCE 303 AA; 34194 MW; D165659BFAB9C40D CRC64;

    MIAMLTVLLY LALILEPRTA VQAGHLPKPI IWAEPGSVIA AYTSVIIWCW

    GSWEAQYYYL DKEKSVNPWD TEVPLENRNK TKFKIRFMTA SYAGIYNCYY

    KSAAGFSEHS DAMELVMTGA YENPSLSVYP SSNVTSGVSI SFKCSSSTLF

    GRFILIQEGK HGLSWTLDSQ HQANQPTHAT FVLDAVAPNH NGTFRCYGFF

    RNEPQVWSKP SNSLDLMISE TKEQSCTPTE DGLETYQKIL IGVLVSFLLL

    FFLLLFLILI GYQCRHKNKA NASVKNTQSE DNAELNSWNP QNEDPPRELC

    TPR

  • UCSC uc007fam.2; mouse.;



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