Mouse Gp49a ELISA Kit

Alternative name
Mouse gp49 ELISA Kit;Mouse Gp49a ELISA Kit;Mouse leukocyte immunoglobulin-like receptor, subfamily B, member 4B ELISA Kit;Mouse mast cell surface glycoprotein Gp49A ELISA Kit;Mouse glycoprotein 49 A ELISA Kit;
Catalog
E059529
species
Mouse
GeneGp49a
SpecificityThis assay recognizes recombinant and natural mouse Mast cell surface glycoprotein Gp49A. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Gp49a ELISA Kit allows for the in vitro quantitative determination of Gp49a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mast cell surface glycoprotein Gp49A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mast cell surface glycoprotein Gp49A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mast cell surface glycoprotein Gp49A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mast cell surface glycoprotein Gp49A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Mast cell surface glycoprotein Gp49A Protein information
Uniprot ID GP49A_MOUSE
Uniprot AC Q61450;
UniGene Mm.439661;
GeneID 14727
KEGG mmu:14727;
Mouse Mast cell surface glycoprotein Gp49A Protein SEQUENCE
SEQUENCE 303 AA; 34194 MW; D165659BFAB9C40D CRC64;
MIAMLTVLLY LALILEPRTA VQAGHLPKPI IWAEPGSVIA AYTSVIIWCW
GSWEAQYYYL DKEKSVNPWD TEVPLENRNK TKFKIRFMTA SYAGIYNCYY
KSAAGFSEHS DAMELVMTGA YENPSLSVYP SSNVTSGVSI SFKCSSSTLF
GRFILIQEGK HGLSWTLDSQ HQANQPTHAT FVLDAVAPNH NGTFRCYGFF
RNEPQVWSKP SNSLDLMISE TKEQSCTPTE DGLETYQKIL IGVLVSFLLL
FFLLLFLILI GYQCRHKNKA NASVKNTQSE DNAELNSWNP QNEDPPRELC
TPR
UCSC uc007fam.2; mouse.;

Category

Mouse Gp49a ELISA Kit

Mouse gp49 ELISA Kit;Mouse Gp49a ELISA Kit;Mouse leukocyte immunoglobulin-like receptor, subfamily B, member 4B ELISA Kit;Mouse mast cell surface glycoprotein Gp49A ELISA Kit;Mouse glycoprotein 49 A ELISA Kit;