Mouse Mapk14 ELISA Kit

Alternative name
Mouse MAP kinase 14 ELISA Kit;Mouse MAPK 14 ELISA Kit;Mouse Crk1 ELISA Kit;Mouse mitogen-activated protein kinase p38 alpha ELISA Kit;Mouse MAP kinase p38 alpha ELISA Kit;Mouse Csbp1 ELISA Kit;Mouse CSBP2 ELISA Kit;Mouse Mxi2 ELISA Kit;Mouse PRKM14 ELISA Kit;Mouse PRKM15 ELISA Kit;Mouse p38 ELISA Kit;Mouse p38-alpha ELISA Kit;Mouse p38MAPK ELISA Kit;Mouse p38a ELISA Kit;Mouse p38alpha ELISA Kit;Mouse mitogen-activated protein kinase 14 ELISA Kit;Mouse cytokine suppressive anti-inflammatory drug binding protein 1 ELISA Kit;Mouse p38 MAP kinase alpha ELISA Kit;Mouse p38 MAPK ELISA Kit;Mouse p38 alpha ELISA Kit;Mouse tRNA synthetase cofactor p38 ELISA Kit;
Catalog
E059502
species
Mouse
GeneMapk14
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse MAPK14. No significant cross-reactivity or interference between Mouse MAPK14 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse MAPK14 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.75ng/mL
Intended UseMouse Mapk14 ELISA Kit allows for the in vitro quantitative determination of Mapk14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MAPK14 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MAPK14. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse MAPK14 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MAPK14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse MAPK14. You can calculate the concentration of Mouse MAPK14 in the samples by comparing the OD of the samples to the standard curve.
Mouse Mitogen-activated protein kinase 14 Protein information
Uniprot ID MK14_MOUSE
Uniprot AC Q9QZ80;
UniGene Mm.311337;
GeneID 26416
KEGG mmu:26416;
Mouse Mitogen-activated protein kinase 14 Protein SEQUENCE
SEQUENCE 360 AA; 41287 MW; DFB03EBCE97BB51A CRC64;
MSQERPTFYR QELNKTIWEV PERYQNLSPV GSGAYGSVCA AFDTKTGHRV
AVKKLSRPFQ SIIHAKRTYR ELRLLKHMKH ENVIGLLDVF TPARSLEEFN
DVYLVTHLMG ADLNNIVKCQ KLTDDHVQFL IYQILRGLKY IHSADIIHRD
LKPSNLAVNE DCELKILDFG LARHTDDEMT GYVATRWYRA PEIMLNWMHY
NQTVDIWSVG CIMAELLTGR TLFPGTDHID QLKLILRLVG TPGAELLKKI
SSESARNYIQ SLAQMPKMNF ANVFIGANPL AVDLLEKMLV LDSDKRITAA
QALAHAYFAQ YHDPDDEPVA DPYDQSFESR DLLIDEWKSL TYDEVISFVP
PPLDQEEMES
UCSC uc008brl.2; mouse. [P47811-1];

Category

Mouse Mapk14 ELISA Kit