Human RCAN1 ELISA Kit

Alternative name
Human ADAPT78 ELISA Kit;Human Down syndrome critical region protein 1 ELISA Kit;Human myocyte-enriched calcineurin-interacting protein 1 ELISA Kit;Human MCIP1 ELISA Kit;Human regulator of calcineurin 1 ELISA Kit;Human CSP1 ELISA Kit;Human DSC1 ELISA Kit;Human DSCR1 ELISA Kit;Human RCN1 ELISA Kit;Human calcipressin-1 ELISA Kit;Human Down syndrome candidate region 1 ELISA Kit;Human Down syndrome critical region gene 1 ELISA Kit;Human calcium and oxidant-inducible mRNA ELISA Kit;Human modulatory calcineurin-interacting protein 1 ELISA Kit;Human near DSCR proline-rich protein ELISA Kit;
Catalog
E005945
species
Human
GeneRCAN1
SpecificityThis assay has high sensitivity and excellent specificity for detection of RCAN1. No significant cross-reactivity or interference between RCAN1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RCAN1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman RCAN1 ELISA Kit allows for the in vitro quantitative determination of RCAN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the assay: RCAN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RCAN1 antibody and an RCAN1-HRP conjugate. The assay sample and buffer are incubated together with RCAN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RCAN1 concentration since RCAN1 from samples and RCAN1-HRP conjugate compete for the anti-RCAN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by RCAN1 from the sample, fewer sites are left to bind RCAN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RCAN1 concentration in each sample is interpolated from this standard curve.
Human Calcipressin-1 Protein information
Uniprot ID RCAN1_HUMAN
Uniprot AC Q9BU69; Q9UF15; Q9UME4;
UniGene Hs.282326;
GeneID 1827
KEGG hsa:1827;
Human Calcipressin-1 Protein SEQUENCE
SEQUENCE 252 AA; 28079 MW; 0B17133D03BD7AFA CRC64;
MEDGVAGPQL GAAAEAAEAA EARARPGVTL RPFAPLSGAA EADEGGGDWS
FIDCEMEEVD LQDLPSATIA CHLDPRVFVD GLCRAKFESL FRTYDKDITF
QYFKSFKRVR INFSNPFSAA DARLQLHKTE FLGKEMKLYF AQTLHIGSSH
LAPPNPDKQF LISPPASPPV GWKQVEDATP VINYDLLYAI SKLGPGEKYE
LHAATDTTPS VVVHVCESDQ EKEEEEEMER MRRPKPKIIQ TRRPEYTPIH
LS
UCSC uc002yuc.5; human. [P53805-1];

Human RCAN1 ELISA Kit

Contact Us