Human RCAN1 ELISA Kit

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  • Alternative name

    Human ADAPT78 ELISA Kit;Human Down syndrome critical region protein 1 ELISA Kit;Human myocyte-enriched calcineurin-interacting protein 1 ELISA Kit;Human MCIP1 ELISA Kit;Human regulator of calcineurin 1 ELISA Kit;Human CSP1 ELISA Kit;Human DSC1 ELISA Kit;Human DSCR1 ELISA Kit;Human RCN1 ELISA Kit;Human calcipressin-1 ELISA Kit;Human Down syndrome candidate region 1 ELISA Kit;Human Down syndrome critical region gene 1 ELISA Kit;Human calcium and oxidant-inducible mRNA ELISA Kit;Human modulatory calcineurin-interacting protein 1 ELISA Kit;Human near DSCR proline-rich protein ELISA Kit;

  • Catalog
    E005945
  • species
    Human
  • GeneRCAN1
  • Standard CurveHuman RCAN1 ELISA Kit
  • Other Species Mouse Rcan1 ELISA KitBovine RCAN1 ELISA KitHamster RCAN1 ELISA KitRat Rcan1 ELISA KitSheep RCAN1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RCAN1. No significant cross-reactivity or interference between RCAN1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RCAN1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman RCAN1 ELISA Kit allows for the in vitro quantitative determination of RCAN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Principle of the assay: RCAN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RCAN1 antibody and an RCAN1-HRP conjugate. The assay sample and buffer are incubated together with RCAN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RCAN1 concentration since RCAN1 from samples and RCAN1-HRP conjugate compete for the anti-RCAN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by RCAN1 from the sample, fewer sites are left to bind RCAN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RCAN1 concentration in each sample is interpolated from this standard curve.
  • Human Calcipressin-1 Protein information
  • Uniprot ID RCAN1_HUMAN
  • Uniprot AC Q9BU69; Q9UF15; Q9UME4;
  • UniGene Hs.282326;
  • GeneID 1827
  • KEGG hsa:1827;
  • Human Calcipressin-1 Protein SEQUENCE
  • SEQUENCE 252 AA; 28079 MW; 0B17133D03BD7AFA CRC64;

    MEDGVAGPQL GAAAEAAEAA EARARPGVTL RPFAPLSGAA EADEGGGDWS

    FIDCEMEEVD LQDLPSATIA CHLDPRVFVD GLCRAKFESL FRTYDKDITF

    QYFKSFKRVR INFSNPFSAA DARLQLHKTE FLGKEMKLYF AQTLHIGSSH

    LAPPNPDKQF LISPPASPPV GWKQVEDATP VINYDLLYAI SKLGPGEKYE

    LHAATDTTPS VVVHVCESDQ EKEEEEEMER MRRPKPKIIQ TRRPEYTPIH

    LS

  • UCSC uc002yuc.5; human. [P53805-1];


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