Mouse MHCDRb1 ELISA Kit

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  • Alternative name

    HLA class II histocompatibility antigen, DRB1-15 beta chain ELISA KIT; DW2.2/DR2.2 ELISA KIT; MHC class II antigen DRB1*15HLA-DRB1 ELISA KIT; HLA-DRB2 ELISA KIT

  • Catalog
    E059358
  • species
    Mouse
  • GeneMHCDRb1
  • Standard CurveMouse MHCDRb1 ELISA Kit
  • Other Species Human MHCDRb1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1). No significant cross-reactivity or interference between Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 12pg/mL.
  • Intended UseMouse MHCDRb1 ELISA Kit allows for the in vitro quantitative determination of MHCDRb1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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