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Mouse MSP ELISA Kit

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  • Alternative name

    Hepatocyte growth factor-like protein ELISA KIT; Macrophage stimulatory protein ELISA KIT; Macrophage-stimulating protein ELISA KIT; MSPMST1 ELISA KIT; D3F15S2 ELISA KIT; DNF15S2 ELISA KIT; HGFL ELISA KIT; MSP ELISA KIT
  • Catalog
    E059306
  • species
    Mouse
  • GeneMSP
  • Standard CurveMouse MSP ELISA Kit
  • Other Species Human MSP ELISA KitHuman MSP-1 ELISA KitMouse MSP/MST1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Macrophage Stimulating Protein (MSP). No significant cross-reactivity or interference between Macrophage Stimulating Protein (MSP) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 1.47pg/mL.
  • Intended UseMouse MSP ELISA Kit allows for the in vitro quantitative determination of MSP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Stimulating Protein (MSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Stimulating Protein (MSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Stimulating Protein (MSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Stimulating Protein (MSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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