Mouse MIP3a ELISA Kit

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  • Alternative name

    C-C motif chemokine 20 ELISA KIT; Beta-chemokine exodus-1 ELISA KIT; CC chemokine LARC ELISA KIT; Liver and activation-regulated chemokine ELISA KIT; Macrophage inflammatory protein 3 alpha ELISA KIT; MIP-3-alphaCCL20 ELISA KIT; LARC ELISA KIT; MIP3A ELISA KIT; SCYA20 ELISA KIT; MIP-3-alpha ELISA KIT

  • Catalog
    E059279
  • species
    Mouse
  • GeneMIP3a
  • Standard CurveMouse MIP3a ELISA Kit
  • Other Species Human MIP3a/CCL20 ELISA KitHuman MIP3a ELISA KitMouse MIP3a/CCL20 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 3 Alpha (MIP3a). No significant cross-reactivity or interference between Macrophage Inflammatory Protein 3 Alpha (MIP3a) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 2.8pg/mL.
  • Intended UseMouse MIP3a ELISA Kit allows for the in vitro quantitative determination of MIP3a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 3 Alpha (MIP3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 3 Alpha (MIP3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.




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