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  • Alternative name

    Calbindin ELISA KIT; Calbindin D28 ELISA KIT; D-28K ELISA KIT; Vitamin D-dependent calcium-binding protein, avian-typeCALB1 ELISA KIT; CAB27 ELISA KIT

  • Catalog
  • species
  • GeneCALB
  • Standard CurveHuman CALB ELISA Kit
  • Other Species Mouse CALB ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Calbindin (CALB). No significant cross-reactivity or interference between Calbindin (CALB) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 4.9pg/mL.
  • Intended UseHuman CALB ELISA Kit allows for the in vitro quantitative determination of CALB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calbindin (CALB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calbindin (CALB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calbindin (CALB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calbindin (CALB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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